PUBLICATION

Innervation modulates the functional connectivity between pancreatic endocrine cells

Authors
Yang, Y.H.C., Briant, L.J.B., Raab, C.A., Mullapudi, S.T., Maischein, H.M., Kawakami, K., Stainier, D.Y.R.
ID
ZDB-PUB-220405-22
Date
2022
Source
eLIFE   11: (Journal)
Registered Authors
Kawakami, Koichi, Maischein, Hans-Martin, Stainier, Didier, Yang, Yu Hsuan Carol
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Animals
  • Endocrine Cells*
  • Zebrafish
  • Insulin-Secreting Cells*
  • Islets of Langerhans*/metabolism
  • Pancreas
(all 6)
PubMed
35373736 Full text @ Elife
Abstract
The importance of pancreatic endocrine cell activity modulation by autonomic innervation has been debated. To investigate this question, we established an in vivo imaging model that also allows chronic and acute neuromodulation with genetic and optogenetic tools. Using the GCaMP6s biosensor together with endocrine cell fluorescent reporters, we imaged calcium dynamics simultaneously in multiple pancreatic islet cell types in live animals in control states and upon changes in innervation. We find that by 4 days post fertilization in zebrafish, a stage when islet architecture is reminiscent of that in adult rodents, prominent activity coupling between beta cells is present in basal glucose conditions. Furthermore, we show that both chronic and acute loss of nerve activity result in diminished beta-beta and alpha-beta activity coupling. Pancreatic nerves are in contact with all islet cell types, but predominantly with beta and delta cells. Surprisingly, a subset of delta cells with detectable peri-islet neural activity coupling had significantly higher homotypic coupling with other delta cells suggesting that some delta cells receive innervation that coordinates their output. Overall, these data show that innervation plays a vital role in the maintenance of homotypic and heterotypic cellular connectivity in pancreatic islets, a process critical for islet function.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
bns162TgTransgenic Insertion
    bns529TgTransgenic Insertion
      gz19TgTransgenic Insertion
        ia1TgTransgenic Insertion
          icm21TgTransgenic Insertion
            jh63TgTransgenic Insertion
              nkgsaizgffm1121AGtTransgenic Insertion
                nkUAShspzGCaMP6s13aTgTransgenic Insertion
                  psi1TgTransgenic Insertion
                    s843TgTransgenic Insertion
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      mScarletEFGmScarlet
                      BoTxBLCEFGBoTxBLC
                      CFPEFGCFP
                      CreEFGCre
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GAL4FFEFGGAL4FF
                      GCaMPEFGGCaMP
                      GFPEFGGFP
                      MCardinalEFGMCardinal
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                      Mapping
                      No data available
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                      Citation
                      Yang, Y.H.C., Briant, L.J.B., Raab, C.A., Mullapudi, S.T., Maischein, H.M., Kawakami, K., Stainier, D.Y.R. (2022) Innervation modulates the functional connectivity between pancreatic endocrine cells. eLIFE. 11:.