PUBLICATION

Sequential action of jnk genes establishes the embryonic left-right axis

Authors
Derrick, C.J., Santos-Ledo, A., Eley, L., Henderson, D.J., Chaudhry, B.
ID
ZDB-PUB-220331-13
Date
2022
Source
Development (Cambridge, England)   149(9): (Journal)
Registered Authors
Chaudhry, Bill, Derrick, Chris
Keywords
Cilia, Jnk, Kupffer's Vesicle, Laterality, PCP, Zebrafish
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Cilia/metabolism
  • Gene Expression Regulation, Developmental
  • Mesoderm/metabolism
  • Body Patterning*/genetics
  • Zebrafish*
  • Animals
(all 8)
PubMed
35352808 Full text @ Development
Abstract
The establishment of the left-right axis is critical for the placement, morphogenesis and function of internal organs. Left-right specification is proposed to be dependent on cilia-driven fluid flow in the embryonic node. Planar cell polarity (PCP) signalling is critical for patterning of nodal cilia, yet downstream effectors driving this process remain elusive. We have examined the role of the JNK gene family, a proposed downstream component of PCP signalling, in the development and function of the zebrafish node. We show jnk1 and jnk2 specify length of nodal cilia, generate flow in the node and restrict Nodal to the left lateral plate mesoderm. Moreover, loss of asymmetric Nodal expression does not result in disturbances to asymmetric organ placement, supporting a model that nodal flow may be dispensable for organ laterality. Later, jnk3 is required to restrict pitx2c expression to the left side and permit correct endodermal organ placement. This work uncovers multiple roles for the JNK gene family acting at different points during left-right axis establishment. It highlights extensive redundancy and indicates JNK activity is distinct from the PCP signalling pathway.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
n2
    Small Deletion
    n3
      Small Deletion
      n4
        Small Deletion
        n5
          Small Deletion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          mapk9CRISPR1-mapk9CRISPR
          mapk10CRISPR1-mapk10CRISPR
          1 - 2 of 2
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          Fish
          Antibodies
          Orthology
          Engineered Foreign Genes
          No data available
          Mapping