PUBLICATION

Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina

Authors
Martins, R.R., Zamzam, M., Tracey-White, D., Moosajee, M., Thummel, R., Henriques, C.M., MacDonald, R.B.
ID
ZDB-PUB-220323-36
Date
2022
Source
Aging Cell   21(4): e13597 (Journal)
Registered Authors
Thummel, Ryan
Keywords
Müller glia, Zebrafish, ageing, degeneration, proliferation, regeneration, retina, telomerase
MeSH Terms
  • Neuroglia/metabolism
  • Zebrafish*
  • Retina*/metabolism
  • Animals, Genetically Modified
  • Ependymoglial Cells
  • Cell Proliferation/physiology
  • Animals
(all 7)
PubMed
35315590 Full text @ Aging Cell
Abstract
Ageing is a significant risk factor for degeneration of the retina. Müller glia cells (MG) are key for neuronal regeneration, so harnessing the regenerative capacity of MG in the retina offers great promise for the treatment of age-associated blinding conditions. Yet, the impact of ageing on MG regenerative capacity is unclear. Here, we show that the zebrafish retina undergoes telomerase-independent, age-related neurodegeneration but that this is insufficient to stimulate MG proliferation and regeneration. Instead, age-related neurodegeneration is accompanied by MG morphological aberrations and loss of vision. Mechanistically, yes-associated protein (Yap), part of the Hippo signalling, has been shown to be critical for the regenerative response in the damaged retina, and we show that Yap expression levels decline with ageing. Despite this, morphologically and molecularly altered aged MG retain the capacity to regenerate neurons after acute light damage, therefore, highlighting key differences in the MG response to high-intensity acute damage versus chronic neuronal loss in the zebrafish retina.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    hu3430
      		Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Martins, R.R., Zamzam, M., Tracey-White, D., Moosajee, M., Thummel, R., Henriques, C.M., MacDonald, R.B. (2022) Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina. Aging Cell. 21(4):e13597.