PUBLICATION

IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish

Authors
Hong, J., Lee, J.G., Sohn, K.C., Lee, K., Lee, S., Lee, J., Hong, J., Choi, D., Hong, Y., Jin, H.S., Choi, D.K., Lee, S.U., Kee, Y., Jung, J., Bae, Y.K., Hwang, R.H., Hur, G.M., Lee, J.S., Ro, H.
ID
ZDB-PUB-211221-4
Date
2021
Source
Communications biology   4: 1405 (Journal)
Registered Authors
Bae, Young Ki, Kee, Yun, Lee, Jeong-Soo, Lee, Seoee, Ro, Hyunju
Keywords
none
MeSH Terms
  • Animals, Genetically Modified/genetics*
  • Genes, Switch*
  • Transgenes*
  • Animals
  • Gene Transfer Techniques*
  • Zebrafish/genetics*
(all 6)
PubMed
34916605 Full text @ Commun Biol
Abstract
Though various transgene expression switches have been adopted in a wide variety of organisms for basic and biomedical research, intrinsic obstacles of those existing systems, including toxicity and silencing, have been limiting their use in vertebrate transgenesis. Here we demonstrate a novel QF-based binary transgene switch (IQ-Switch) that is relatively free of driver toxicity and transgene silencing, and exhibits potent and highly tunable transgene activation by the chemical inducer tebufenozide, a non-toxic lipophilic molecule to developing zebrafish with negligible background. The interchangeable IQ-Switch makes it possible to elicit ubiquitous and tissue specific transgene expression in a spatiotemporal manner. We generated a RASopathy disease model using IQ-Switch and demonstrated that the RASopathy symptoms were ameliorated by the specific BRAF(V600E) inhibitor vemurafenib, validating the therapeutic use of the gene switch. The orthogonal IQ-Switch provides a state-of-the-art platform for flexible regulation of transgene expression in zebrafish, potentially applicable in cell-based systems and other model organisms.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
GAL4EFGGAL4
mCherryEFGmCherry
QFEFGQF
ZsGreenEFGZsGreen
1 - 5 of 5
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Mapping
No data available