PUBLICATION

A Comparative Analysis of Gene and Protein Expression Throughout a Full 28-Day Retinal Regeneration Time-Course in Adult Zebrafish

Authors
Kramer, A.C., Gurdziel, K., Thummel, R.
ID
ZDB-PUB-211119-2
Date
2021
Source
Frontiers in cell and developmental biology   9: 741514 (Journal)
Registered Authors
Thummel, Ryan
Keywords
3′RNA-seq, Müller glia, gliosis, regeneration, retina, stem cell
Datasets
GEO:GSE180518
MeSH Terms
none
PubMed
34790663 Full text @ Front Cell Dev Biol
Abstract
Following photoreceptors ablation by intense light exposure, adult zebrafish are capable of complete regeneration due to the ability of their Müller glia (MG) to re-enter the cell cycle, creating progenitors that differentiate into new photoreceptors. The majority of previous reports on retinal regeneration focused on the first few days of the regenerative response, which include MG cell-cycle re-entry and progenitor cell proliferation. With this study, we analyzed the full 28-day time-course of regeneration by pairing a detailed morphological/immunological analysis with RNA-seq transcriptional profiling at 8 key time points during retinal regeneration. We observed several novel findings. First, we provide evidence for two separate peaks of MG gliosis, with the secondary gliotic peak occurring after MG cell-cycle re-entry. Second, we highlight a distinct transcriptional shift between 5- and 10-days post lesion that highlights the transition from progenitor proliferation to differentiation into new photoreceptors. Third, we show distinctly different patterns of transcriptional recovery of the photoreceptor opsins at 28 days post lesion. Finally, using differential gene expression analysis, we revealed that the established functional recovery of the retina at 28 days post lesion does not, in fact, return to an undamaged transcriptional state, potentially redefining what the field considers complete regeneration. Together, to our knowledge, this work represents the first histological and transcriptomic map of a 28-day time-course of retinal regeneration in adult zebrafish.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-opn1lw1polyclonalRabbit
    Ab1-opn1mw1polyclonalRabbit
    Ab1-opn1sw1polyclonalRabbit
    Ab1-opn1sw2polyclonalRabbit
    Ab2-rhopolyclonalRabbit
    Ab-4C4monoclonal
      		IgG1Mouse
      1 - 6 of 6
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      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Kramer, A.C., Gurdziel, K., Thummel, R. (2021) A Comparative Analysis of Gene and Protein Expression Throughout a Full 28-Day Retinal Regeneration Time-Course in Adult Zebrafish. Frontiers in cell and developmental biology. 9:741514.