PUBLICATION

The broad role of Nkx3.2 in the development of the zebrafish axial skeleton

Authors
Waldmann, L., Leyhr, J., Zhang, H., Öhman-Mägi, C., Allalou, A., Haitina, T.
ID
ZDB-PUB-210820-6
Date
2021
Source
PLoS One   16: e0255953 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Animals
  • Transcription Factors
  • Homeodomain Proteins*
  • Zebrafish*
  • Bone and Bones
(all 6)
PubMed
34411150 Full text @ PLoS One
Abstract
The transcription factor Nkx3.2 (Bapx1) is an important chondrocyte maturation inhibitor. Previous Nkx3.2 knockdown and overexpression studies in non-mammalian gnathostomes have focused on its role in primary jaw joint development, while the function of this gene in broader skeletal development is not fully described. We generated a mutant allele of nkx3.2 in zebrafish with CRISPR/Cas9 and applied a range of techniques to characterize skeletal phenotypes at developmental stages from larva to adult, revealing loss of the jaw joint, fusions in bones of the occiput, morphological changes in the Weberian apparatus, and the loss or deformation of bony elements derived from basiventral cartilages of the vertebrae. Axial phenotypes are reminiscent of Nkx3.2 knockout in mammals, suggesting that the function of this gene in axial skeletal development is ancestral to osteichthyans. Our results highlight the broad role of nkx3.2 in zebrafish skeletal development and its context-specific functions in different skeletal elements.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
1 - 10 of 65
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba2TgTransgenic Insertion
    uu2803
      Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      nkx3-2CRISPR6-nkx3-2CRISPR
      nkx3-2CRISPR7-nkx3-2CRISPR
      1 - 2 of 2
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      Fish
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
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      Mapping
      No data available