PUBLICATION

A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle

Authors
Yang, Y., Wang, H., He, J., Shi, W., Jiang, Z., Gao, L., Jiang, Y., Ni, R., Yang, Q., Luo, L.
ID
ZDB-PUB-210624-7
Date
2021
Source
Proceedings of the National Academy of Sciences of the United States of America   118(25): (Journal)
Registered Authors
Luo, Lingfei
Keywords
cell cycle, endoderm, fate map, pancreatic fate, single-cell labeling and tracing
MeSH Terms
  • Zebrafish Proteins/metabolism
  • Cell Lineage
  • Stem Cells/cytology*
  • Single-Cell Analysis*
  • Animals
  • G1 Phase
  • Zebrafish/embryology*
  • Endoderm/cytology*
  • Pancreas/cytology*
  • Cell Proliferation
  • Hedgehog Proteins/metabolism
  • Signal Transduction
  • Cell Cycle*
(all 13)
PubMed
34161274 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell-resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at single-cell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-cdk2standard conditionsPrim-5
AB + MO1-cdk2standard conditionsPrim-5
AB + MO1-shhastandard conditionsPrim-5
AB + MO1-shhastandard conditionsPrim-5
AB + MO1-shha + MO2-gmnnstandard conditionsPrim-5
AB + MO1-shha + MO2-gmnnstandard conditionsPrim-5
AB + MO2-gmnnstandard conditionsPrim-5
AB + MO2-gmnnstandard conditionsPrim-5
AB + MO2-gmnnstandard conditionsPrim-5
AB + MO2-gmnnstandard conditionsPrim-5
1 - 10 of 55
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq40TgTransgenic Insertion
    cq84
      		Point Mutation
      cq109TgTransgenic Insertion
        cq110TgTransgenic Insertion
          jh1TgTransgenic Insertion
            s870TgTransgenic Insertion
              w30TgTransgenic Insertion
                w141TgTransgenic Insertion
                  zf5TgTransgenic Insertion
                    1 - 9 of 9
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    bmp2bMO3-bmp2bMRPHLNO
                    cdk2MO1-cdk2MRPHLNO
                    gmnnMO2-gmnnMRPHLNO
                    shhaMO1-shhaMRPHLNO
                    1 - 4 of 4
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                    Fish
                    Fish
                    AB
                    AB + MO1-cdk2
                    AB + MO1-shha
                    AB + MO1-shha + MO2-gmnn
                    AB + MO2-gmnn
                    AB + MO3-bmp2b
                    cq40Tg/cq40Tg
                    cq40Tg/cq40Tg + MO2-gmnn
                    cq109Tg/cq109Tg
                    cq109Tg/cq109Tg; cq40Tg/cq40Tg
                    1 - 10 of 26
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                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    CeruleanEFGCerulean
                    EGFPEFGEGFP
                    GFPEFGGFP
                    KikGREFGKikGR
                    mCherryEFGmCherry
                    1 - 5 of 5
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                    Mapping
                    No data available
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                    Citation
                    Yang, Y., Wang, H., He, J., Shi, W., Jiang, Z., Gao, L., Jiang, Y., Ni, R., Yang, Q., Luo, L. (2021) A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle. Proceedings of the National Academy of Sciences of the United States of America. 118(25):.