PUBLICATION

The development of zebrafish pancreas affected by deficiency of Hedgehog signaling

Authors

Korzh, S., Winata, C.L., Gong, Z., Korzh, V.

ID

ZDB-PUB-210605-13

Date

2021

Source

Gene expression patterns : GEP 41: 119185 (Journal)

Registered Authors

Gong, Zhiyuan, Korzh, Svitlana, Korzh, Vladimir, Winata, Cecilia Lanny

Keywords

annular pancreas, elastase, green fluorescent protein (GFP), pancreas, transgenic, zebrafish

MeSH Terms

Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Signal Transduction*
Smoothened Receptor/genetics
Smoothened Receptor/metabolism*
Zebrafish*/genetics
Hedgehog Proteins*/genetics
Pancreas
Animals

(all 9)

PubMed

34087472 Full text @ Gene Expr. Patterns

Abstract

The pancreas development depends on complex regulation of several signaling pathways, including the Hedgehog (Hh) signaling via a receptor complex component, Smoothened, which deficiency blocks the Hh signaling. Such a defect in birds and mammals results in an annular pancreas. We showed that in developing zebrafish, the mutation of Smoothened or inhibition of Hh signaling by its antagonist cyclopamine caused developmental defects of internal organs, liver, pancreas, and gut. In particular, the pancreatic primordium was duplicated. The two exocrine pancreatic primordia surround the gut. This phenomenon correlates with a significant reduction of the gut's diameter, causing the annular pancreas phenotype.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
DsRed	gz15Tg	standard conditions	Day 4	liver	IFL	Fig. 2
DsRed	smo^b641/b641; gz15Tg	standard conditions	Day 4	liver	IFL	Fig. 2
EGFP	gz15Tg	standard conditions	Day 4	exocrine pancreas	IFL	Fig. 2
EGFP	gz2Tg	standard conditions	Protruding-mouth	pancreas	IFL	Fig. 1
EGFP	gz2Tg	standard conditions	Day 4	pancreas	IFL	Fig. 1
EGFP	smo^b641/b641; gz15Tg	standard conditions	Day 4	exocrine pancreas	IFL	Fig. 2
EGFP	smo^b641/b641; gz2Tg	standard conditions	Protruding-mouth	pancreas	IFL	Fig. 1
EGFP	smo^b641/b641; gz2Tg	standard conditions	Day 4	pancreas	IFL	Fig. 1
ela3l	WT	control	Day 6	exocrine pancreas	ISH	Fig. 3
ela3l	WT	chemical treatment by environment: Cyclopamine	Day 6	exocrine pancreas	ISH	Fig. 3

1 - 10 of 18

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment by environment: Cyclopamine	Day 6	exocrine pancreas ela3l expression spatial pattern, abnormal	Fig. 3
WT	chemical treatment by environment: Cyclopamine	Day 6	gut decreased diameter, abnormal	Fig. 3
WT	chemical treatment by environment: Cyclopamine	Day 6	pancreas duplicated, abnormal	Fig. 3
s854Tg	chemical treatment by environment: Cyclopamine	Pec-fin	pancreas duplicated, abnormal	Fig. 2
s854Tg	chemical treatment by environment: Cyclopamine	Pec-fin	pancreas GFP expression spatial pattern, abnormal	Fig. 2
smo^b641/b641; gz15Tg	standard conditions	Day 4	exocrine pancreas EGFP expression spatial pattern, abnormal	Fig. 2
smo^b641/b641; gz15Tg	standard conditions	Day 4	gut decreased diameter, abnormal	Fig. 2
smo^b641/b641; gz15Tg	standard conditions	Day 4	liver DsRed expression spatial pattern, abnormal	Fig. 2
smo^b641/b641; gz15Tg	standard conditions	Day 4	liver duplicated, abnormal	Fig. 2
smo^b641/b641; gz15Tg	standard conditions	Day 4	pancreas duplicated, abnormal	Fig. 2

1 - 10 of 14

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b641		Point Mutation	smo
gz2Tg	Tg(ela3l:EGFP)	Transgenic Insertion
gz15Tg	Tg(ela3l:EGFP) Tg(fabp10a:DsRed)	Transgenic Insertion
s854Tg	Tg(Xla.Eef1a1:GFP)	Transgenic Insertion

1 - 4 of 4

Show

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
DsRed	EFG	DsRed
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

Korzh et al., 2021 ZDB-PUB-210605-13 PMID:34087472

The development of zebrafish pancreas affected by deficiency of Hedgehog signaling

Korzh et al., 2021

ZDB-PUB-210605-13
PMID:34087472