PUBLICATION

Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish

Authors
Ceriani, R., Whitlock, K.E.
ID
ZDB-PUB-210603-36
Date
2021
Source
International Journal of Molecular Sciences   22(11): (Journal)
Registered Authors
Ceriani, Ricardo
Keywords
BrdU, Pre-optic area (POA), cytoplasmic Sox2, tanycyte, testosterone
MeSH Terms
  • Neurons/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Glial Fibrillary Acidic Protein/genetics
  • Vimentin/genetics
  • Gonadotropin-Releasing Hormone/genetics*
  • Ependymoglial Cells/metabolism
  • Neurogenesis/genetics*
  • SOX Transcription Factors/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Animals
  • Hypothalamus/growth & development
  • Hypothalamus/metabolism*
(all 14)
PubMed
34072957 Full text @ Int. J. Mol. Sci.
Abstract
Recently, it has been shown in adult mammals that the hypothalamus can generate new cells in response to metabolic changes, and tanycytes, putative descendants of radial glia, can give rise to neurons. Previously we have shown in vitro that neurospheres generated from the hypothalamus of adult zebrafish show increased neurogenesis in response to exogenously applied hormones. To determine whether adult zebrafish have a hormone-responsive tanycyte-like population in the hypothalamus, we characterized proliferative domains within this region. Here we show that the parvocellular nucleus of the preoptic region (POA) labels with neurogenic/tanycyte markers vimentin, GFAP/Zrf1, and Sox2, but these cells are generally non-proliferative. In contrast, Sox2+ proliferative cells in the ventral POA did not express vimentin and GFAP/Zrf1. A subset of the Sox2+ cells co-localized with Fezf2:GFP, a transcription factor important for neuroendocrine cell specification. Exogenous treatments of GnRH and testosterone were assayed in vivo. While the testosterone-treated animals showed no significant changes in proliferation, the GnRH-treated animals showed significant increases in the number of BrdU-labeled cells and Sox2+ cells. Thus, cells in the proliferative domains of the zebrafish POA do not express radial glia (tanycyte) markers vimentin and GFAP/Zrf1, and yet, are responsive to exogenously applied GnRH treatment.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s2503TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab2-krtmonoclonal
      		IgG1Mouse
      Ab2-pcnamonoclonalIgG2aMouse
      Ab3-sox2polyclonalIgGRabbit
      Ab5-vimpolyclonal
        		IgYChicken
        Ab10-sox2polyclonal
          		IgGRabbit
          zrf-1monoclonalIgG1Mouse
          1 - 6 of 6
          Show
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          1 - 1 of 1
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          Mapping
          No data available
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          Citation
          Ceriani, R., Whitlock, K.E. (2021) Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish. International Journal of Molecular Sciences. 22(11):.