PUBLICATION

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia

Authors
Kindt, K.S., Akturk, A., Jarysta, A., Day, M., Beirl, A., Flonard, M., Tarchini, B.
ID
ZDB-PUB-210519-24
Date
2021
Source
Nature communications   12: 2861 (Journal)
Registered Authors
Beirl, Alisha, Kindt, Katie
Keywords
none
MeSH Terms
  • Cell Polarity/genetics
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Confocal/methods
  • GTP-Binding Protein alpha Subunits, Gi-Go/genetics
  • GTP-Binding Protein alpha Subunits, Gi-Go/metabolism*
  • Female
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism*
  • Male
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Animals
  • Hair Cells, Auditory/cytology
  • Hair Cells, Auditory/metabolism*
  • Zebrafish/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Epithelium/metabolism*
(all 20)
PubMed
34001891 Full text @ Nat. Commun.
Abstract
Hair cells detect sound, head position or water movements when their mechanosensory hair bundle is deflected. Each hair bundle has an asymmetric architecture that restricts stimulus detection to a single axis. Coordinated hair cell orientations within sensory epithelia further tune stimulus detection at the organ level. Here, we identify GPR156, an orphan GPCR of unknown function, as a critical regulator of hair cell orientation. We demonstrate that the transcription factor EMX2 polarizes GPR156 distribution, enabling it to signal through Gαi and trigger a 180° reversal in hair cell orientation. GPR156-Gαi mediated reversal is essential to establish hair cells with mirror-image orientations in mouse otolith organs in the vestibular system and in zebrafish lateral line. Remarkably, GPR156-Gαi also instructs hair cell reversal in the auditory epithelium, despite a lack of mirror-image organization. Overall, our work demonstrates that conserved GPR156-Gαi signaling is integral to the framework that builds directional responses into mechanosensory epithelia.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
idc1TgTransgenic Insertion
    idc15
      Small Deletion
      sa34566
        Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        gpr156CRISPR1-gpr156CRISPR
        1 - 1 of 1
        Show
        Fish
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab1-emx2polyclonalRabbit
        Ab1-myo7apolyclonal
          Rabbit
          1 - 2 of 2
          Show
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GCaMPEFGGCaMP
          1 - 1 of 1
          Show
          Mapping
          No data available