PUBLICATION

The alx3 gene shapes the zebrafish neurocranium by regulating frontonasal neural crest cell differentiation timing

Authors
Mitchell, J.M., Sucharov, J., Pulvino, A.T., Brooks, E.P., Gillen, A.E., Nichols, J.T.
ID
ZDB-PUB-210321-4
Date
2021
Source
Development (Cambridge, England)   148(7): (Journal)
Registered Authors
Brooks, Elliott, Nichols, James Tucker
Keywords
Bone, Cartilage, Craniofacial skeleton, Differentiation, Neural crest cells, Neurocranium, Zebrafish, alx, scRNA-seq
Datasets
GEO:GSE163826
MeSH Terms
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Transcriptome
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Head
  • Neural Crest/cytology
  • Neural Crest/metabolism*
  • Transcription Factors/genetics
  • Skull/metabolism
  • Cell Differentiation/genetics*
  • Cell Differentiation/physiology
  • Morphogenesis
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
  • Cartilage/metabolism
  • Organogenesis
  • Animals
  • Mice, Transgenic
(all 21)
PubMed
33741714 Full text @ Development
Abstract
During craniofacial development, different populations of cartilage and bone forming cells develop in precise locations in the head. Most of these cells are derived from pluripotent cranial neural crest cells and differentiate with distinct developmental timing and cellular morphologies. The mechanisms that divide neural crest cells into discrete populations are not fully understood. Here we use single-cell RNA sequencing to transcriptomically define different populations of cranial neural crest cells. We discovered that the transcription factor encoding alx gene family is enriched in the frontonasal population of neural crest cells. Genetic mutant analyses indicate that alx3 functions to regulate the distinct differentiation timing and cellular morphologies among frontonasal neural crest cell subpopulations. This study furthers our understanding of how genes controlling developmental timing shape craniofacial skeletal elements.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
co3000
    Small Deletion
    co3001
      Small Deletion
      co3002
        Small Deletion
        dco3003
          Indel
          dco3004
            Indel
            vu234TgTransgenic Insertion
              y1TgTransgenic Insertion
                zc81TgTransgenic Insertion
                1 - 8 of 8
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                alx1CRISPR1-alx1CRISPR
                alx3CRISPR1-alx3CRISPR
                alx3CRISPR2-alx3CRISPR
                alx4aCRISPR1-alx4aCRISPR
                alx4bCRISPR1-alx4bCRISPR
                1 - 5 of 5
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                Fish
                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                mRFPEFGmRFP
                1 - 2 of 2
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                Mapping
                No data available