PUBLICATION

Py3-FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes

Authors
Wang, Z., Mizoguchi, T., Kuribara, T., Nakajima, M., Iwata, M., Sakamoto, Y., Nakamura, H., Murayama, T., Nemoto, T., Itoh, M.
ID
ZDB-PUB-210210-11
Date
2021
Source
Open Biology   11: 200241 (Journal)
Registered Authors
Itoh, Motoyuki, Mizoguchi, Takamasa
Keywords
collagen-rich tissue, ionocyte, live imaging, polypyrrole, zebrafish
MeSH Terms
  • Humans
  • Cartilage/cytology
  • Cartilage/metabolism
  • Fluorescent Dyes/adverse effects
  • Fluorescent Dyes/chemical synthesis*
  • Fluorescent Dyes/pharmacokinetics
  • Fluorescein-5-isothiocyanate/analogs & derivatives*
  • Animals
  • Zebrafish
  • Nylons/chemistry
  • Collagen/metabolism*
  • Cell Line
  • Polymers/chemistry
  • Pyrroles/chemistry
  • Microscopy, Fluorescence/methods
(all 15)
PubMed
33561382 Full text @ Open Biol.
Abstract
Polypyrrole-based polyamides are used as sequence-specific DNA probes. However, their cellular uptake and distribution are affected by several factors and have not been extensively studied in vivo. Here, we generated a series of fluorescence-conjugated polypyrrole compounds and examined their cellular distribution using live zebrafish and cultured human cells. Among the evaluated compounds, Py3-FITC was able to visualize collagen-rich tissues, such as the jaw cartilage, opercle and bulbus arteriosus, in early-stage living zebrafish embryos. Then, we stained cultured human cells with Py3-FITC and found that the staining became more intense as the amount of collagen was increased. In addition, Py3-FITC-stained HR cells, which represent a type of ionocyte on the body surface of living zebrafish embryos. Py3-FITC has low toxicity, and collagen-rich tissues and ionocytes can be visualized when soaked in Py3-FITC solution. Therefore, Py3-FITC may be a useful live imaging tool for detecting changes in collagen-rich tissue and ionocytes, including their mammalian analogues, during both normal development and disease progression.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    ta52b
      		Point Mutation
      um13TgTransgenic Insertion
        w2
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-col2amonoclonal
            		IgG1Mouse
            Ab3-col1a1monoclonal
              		IgG1Mouse
              1 - 2 of 2
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              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              DsRedxEFGDsRedx
              1 - 1 of 1
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              Mapping
              No data available
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              Citation
              Wang, Z., Mizoguchi, T., Kuribara, T., Nakajima, M., Iwata, M., Sakamoto, Y., Nakamura, H., Murayama, T., Nemoto, T., Itoh, M. (2021) Py3-FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes. Open Biology. 11:200241.