PUBLICATION

Nrl Is Dispensable for Specification of Rod Photoreceptors in Adult Zebrafish Despite Its Deeply Conserved Requirement Earlier in Ontogeny

Authors
Oel, A.P., Neil, G.J., Dong, E.M., Balay, S.D., Collett, K., Allison, W.T.
ID
ZDB-PUB-201212-17
Date
2020
Source
iScience   23: 101805 (Journal)
Registered Authors
Allison, Ted, Oel, Phil
Keywords
Biological Sciences, Developmental Biology, Molecular Biology
MeSH Terms
none
PubMed
33299975 Full text @ iScience
Abstract
The transcription factor NRL (neural retina leucine zipper) has been canonized as the master regulator of photoreceptor cell fate in the retina. NRL is necessary and sufficient to specify rod cell fate and to preclude cone cell fate in mice. By engineering zebrafish, we tested if NRL function has conserved roles beyond mammals or beyond nocturnal species, i.e., in a vertebrate possessing a greater and more typical diversity of cone sub-types. Transgenic expression of Nrl from zebrafish or mouse was sufficient to induce rod photoreceptor cells. Zebrafish nrl-/- mutants lacked rods (and had excess UV-sensitive cones) as young larvae; thus, the conservation of Nrl function between mice and zebrafish appears sound. Strikingly, however, rods were abundant in adult nrl-/- null mutant zebrafish. Rods developed in adults despite Nrl protein being undetectable. Therefore, a yet-to-be-revealed non-canonical pathway independent of Nrl is able to specify the fate of some rod photoreceptors.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a131TgTransgenic Insertion
    kj2TgTransgenic Insertion
      kj9TgTransgenic Insertion
        q28TgTransgenic Insertion
          q30TgTransgenic Insertion
            ua3140TgTransgenic Insertion
              ua3162TgTransgenic Insertion
                ua3174TgTransgenic Insertion
                  ua5009
                    Indel
                    ua5014
                      Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      mafbbCRISPR1-mafbbCRISPR
                      mafbbCRISPR2-mafbbCRISPR
                      nrlCRISPR1-nrlCRISPR
                      nrlCRISPR2-nrlCRISPR
                      nrlCRISPR3-nrlCRISPR
                      nrlMO1-nrlMRPHLNO
                      1 - 6 of 6
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                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      CreEFGCre
                      EGFPEFGEGFP
                      mCherryEFGmCherry
                      NTREFGNTR
                      RFPEFGRFP
                      TomatoEFGTomato
                      YFPEFGYFP
                      1 - 8 of 8
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                      Mapping
                      No data available