PUBLICATION

Optogenetic investigation of BMP target gene expression diversity

Authors

Rogers, K.W., ElGamacy, M., Jordan, B.M., Müller, P.

ID

ZDB-PUB-201120-92

Date

2020

Source

eLIFE 9: (Journal)

Registered Authors

Müller, Patrick, Rogers, Katherine

Keywords

cell biology, developmental biology, zebrafish

Datasets

GEO:GSE135100

MeSH Terms

Animals
Bone Morphogenetic Proteins/genetics*
Bone Morphogenetic Proteins/metabolism
Transcription, Genetic
Body Patterning
Zebrafish/embryology
Zebrafish/genetics
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Signal Transduction
Optogenetics*
Nodal Protein/genetics
Nodal Protein/metabolism
Fibroblast Growth Factors/genetics
Fibroblast Growth Factors/metabolism
Gene Expression Regulation, Developmental*
Kinetics

(all 17)

PubMed

33174840 Full text @ Elife

Abstract

Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found that they have diverse spatiotemporal expression patterns. Transcriptional responses to optogenetically delivered high- and low-amplitude BMP signaling pulses indicate that spatiotemporal expression is not fully defined by different BMP signaling activation thresholds. Additionally, we observed negligible correlations between spatiotemporal expression and transcription kinetics for the majority of analyzed genes in response to BMP signaling pulses. In contrast, spatial differences between BMP target genes largely collapsed when FGF and Nodal signaling were inhibited. Our results suggest that, similar to other patterning systems, combinatorial signaling is likely to be a major driver of spatial diversity in BMP-dependent gene expression in zebrafish.

Genes / Markers

Figures