PUBLICATION

Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells

Authors
Lv, P., Ma, D., Gao, S., Zhang, Y., Bae, Y.K., Liang, G., Gao, S., Choi, J.H., Kim, C.H., Wang, L., Liu, F.
ID
ZDB-PUB-200822-23
Date
2020
Source
Stem Cell Reports   15(3): 749-760 (Journal)
Registered Authors
Bae, Young Ki, Choi, Jung-Hwa, Gao, Shuai, Kim, Cheol-Hee, Liu, Feng, Lv, Peng, Ma, Dongyuan, Zhang, Yifan
Keywords
foxn1/Casper mutant, hematopoietic stem cells, nonconditioned cell transplantation, xenograft, zebrafish
MeSH Terms
  • Forkhead Transcription Factors/genetics*
  • Forkhead Transcription Factors/metabolism
  • Mutation/genetics*
  • Allografts/transplantation*
  • Neoplasms/pathology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Base Sequence
  • Heterografts/transplantation*
  • Hematopoietic Stem Cells/cytology
  • Treatment Outcome
  • Hematopoietic Stem Cell Transplantation
  • Fetal Stem Cells/cytology
  • Animals
  • Zebrafish/embryology
  • Zebrafish/genetics*
(all 16)
PubMed
32822590 Full text @ Stem Cell Reports
Abstract
Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic stem cell (HSC) transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization zebrafish embryos produce a better transplant outcome in foxn1/Casper mutants, compared with adult HSCs. In addition to HSCT, the foxn1/Casper mutant is feasible for allografts of myelodysplastic syndrome-like and muscle cells, as well as xenografts of medaka muscle cells. In summary, foxn1/Casper mutants permit the nonconditioned engraftment of multiple cell types and visualized characterization of transplanted cells in vivo.
Genes / Markers
Figures
Figure Gallery (2 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
foxn1ioz112/ioz112standard conditionsDay 5
foxn1ioz112/ioz112standard conditionsDay 5
foxn1ioz112/ioz112standard conditionsDay 5
foxn1ioz112/ioz112standard conditionsDay 5
foxn1ioz112/ioz112standard conditionsDay 5
foxn1ioz112/ioz112standard conditionsAdult
foxn1ioz112/ioz112standard conditionsAdult
foxn1ioz112/ioz112standard conditionsAdult
foxn1ioz112/ioz112standard conditionsAdult
foxn1ioz112/ioz112standard conditionsAdult
1 - 10 of 13
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    ioz112
      		Indel
      la2TgTransgenic Insertion
        pku329TgTransgenic Insertion
          w2
            		Point Mutation
            zf169TgTransgenic Insertion
              zf411TgTransgenic Insertion
                zf737EtTransgenic Insertion
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Fish
                  foxn1ioz112/ioz112
                  foxn1ioz112/+
                  WT
                  1 - 3 of 3
                  Show
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRedEFGDsRed
                  EGFPEFGEGFP
                  GFPEFGGFP
                  1 - 3 of 3
                  Show
                  Mapping
                  No data available
                  Your Input Welcome
                  We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                  Citation
                  Lv, P., Ma, D., Gao, S., Zhang, Y., Bae, Y.K., Liang, G., Gao, S., Choi, J.H., Kim, C.H., Wang, L., Liu, F. (2020) Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells. Stem Cell Reports. 15(3):749-760.