PUBLICATION

Expression of a Barhl1a reporter in subsets of retinal ganglion cells and commissural neurons of the developing zebrafish brain

Authors
Albadri, S., Armant, O., Aljand-Geschwill, T., Del Bene, F., Carl, M., Strähle, U., Poggi, L.
ID
ZDB-PUB-200603-13
Date
2020
Source
Scientific Reports   10: 8814 (Journal)
Registered Authors
Albadri, Shahad, Armant, Olivier, Carl, Matthias, Del Bene, Filippo, Poggi, Lucia, Strähle, Uwe
Keywords
none
MeSH Terms
  • Optic Chiasm/cytology
  • Optic Chiasm/embryology*
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • Gene Expression Regulation, Developmental*
  • DNA-Binding Proteins/physiology
  • Neurons/metabolism
  • Diencephalon/cytology
  • Diencephalon/embryology
  • Genes, Reporter
  • Animals
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
  • Microscopy, Fluorescence
  • Neural Stem Cells/metabolism
  • Axons/ultrastructure
  • Nerve Tissue Proteins/biosynthesis*
  • Intravital Microscopy
  • Cell Lineage
  • Retinal Ganglion Cells/classification
  • Retinal Ganglion Cells/metabolism*
  • Gene Duplication
  • Amacrine Cells/metabolism
(all 26)
PubMed
32483163 Full text @ Sci. Rep.
Abstract
Promoting the regeneration or survival of retinal ganglion cells (RGCs) is one focus of regenerative medicine. Homeobox Barhl transcription factors might be instrumental in these processes. In mammals, only barhl2 is expressed in the retina and is required for both subtype identity acquisition of amacrine cells and for the survival of RGCs downstream of Atoh7, a transcription factor necessary for RGC genesis. The underlying mechanisms of this dual role of Barhl2 in mammals have remained elusive. Whole genome duplication in the teleost lineage generated the barhl1a and barhl2 paralogues. In the Zebrafish retina, Barhl2 functions as a determinant of subsets of amacrine cells lineally related to RGCs independently of Atoh7. In contrast, barhl1a expression depends on Atoh7 but its expression dynamics and function have not been studied. Here we describe for the first time a Barhl1a reporter line in vivo showing that barhl1a turns on exclusively in subsets of RGCs and their post-mitotic precursors. We also show transient expression of barhl1a:GFP in diencephalic neurons extending their axonal projections as part of the post-optic commissure, at the time of optic chiasm formation. This work sets the ground for future studies on RGC subtype identity, axonal projections and genetic specification of Barhl1a-positive RGCs and commissural neurons.
Genes / Markers
Figures
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cu2TgTransgenic Insertion
    ia6TgTransgenic Insertion
      tpl2TgTransgenic Insertion
        uh6TgTransgenic Insertion
          uh7TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GAL4FFEFGGAL4FF
            GFPEFGGFP
            mRFPEFGmRFP
            RFPEFGRFP
            1 - 6 of 6
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            Mapping
            No data available
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            Citation
            Albadri, S., Armant, O., Aljand-Geschwill, T., Del Bene, F., Carl, M., Strähle, U., Poggi, L. (2020) Expression of a Barhl1a reporter in subsets of retinal ganglion cells and commissural neurons of the developing zebrafish brain. Scientific Reports. 10:8814.