PUBLICATION

Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract

Authors
Sidhwani, P., Leerberg, D.M., Boezio, G.L.M., Capasso, T.L., Yang, H., Chi, N.C., Roman, B.L., Stainier, D.Y.R., Yelon, D.
ID
ZDB-PUB-200524-4
Date
2020
Source
Development (Cambridge, England)   147(12): (Journal)
Registered Authors
Chi, Neil C., Leerberg, Dena M., Roman, Beth, Stainier, Didier, Yang, Hongbo, Yelon, Deborah
Keywords
Acvrl1, Cardiac function, Endocardium, Heart development, Outflow tract, Zebrafish
MeSH Terms
  • Activin Receptors/antagonists & inhibitors
  • Activin Receptors/genetics
  • Activin Receptors/metabolism
  • Troponin T/antagonists & inhibitors
  • Troponin T/genetics
  • Troponin T/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Animals
  • Morpholinos/metabolism
  • Endocardium/cytology
  • Endocardium/metabolism*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Heart/anatomy & histology
  • Heart/growth & development
  • Heart/physiology*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Cell Proliferation
(all 23)
PubMed
32439760 Full text @ Development
Abstract
Physical forces are important participants in the cellular dynamics that shape developing organs. During heart formation, for example, contractility and blood flow generate biomechanical cues that influence patterns of cell behavior. Here, we address the interplay between function and form during the assembly of the cardiac outflow tract (OFT), a crucial connection between the heart and vasculature that develops while circulation is underway. In zebrafish, we find that the OFT expands via accrual of both endocardial and myocardial cells. However, when cardiac function is disrupted, OFT endocardial growth ceases, accompanied by reduced proliferation and reduced addition of cells from adjacent vessels. The flow-responsive TGFβ receptor Acvrl1 is required for addition of endocardial cells, but not for their proliferation, indicating distinct modes of function-dependent regulation for each of these essential cell behaviors. Together, our results indicate that cardiac function modulates OFT morphogenesis by triggering endocardial cell accumulation that induces OFT lumen expansion and shapes OFT dimensions; moreover, these morphogenetic mechanisms provide new perspectives regarding the potential causes of cardiac birth defects.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Sidhwani, P., Leerberg, D.M., Boezio, G.L.M., Capasso, T.L., Yang, H., Chi, N.C., Roman, B.L., Stainier, D.Y.R., Yelon, D. (2020) Cardiac function modulates endocardial cell dynamics to shape the cardiac outflow tract. Development (Cambridge, England). 147(12):.