PUBLICATION

Functional Characterization and Expression Analyses Show Differential Roles of Maternal and Zygotic Dgcr8 in Early Embryonic Development

Authors
Zhu, Z., Liu, Y., Xu, W., Liu, T., Xie, Y., Sham, K.W.Y., Sha, O., Cheng, C.H.K.
ID
ZDB-PUB-200422-134
Date
2020
Source
Frontiers in genetics   11: 299 (Journal)
Registered Authors
Keywords
embryonic development, maternal dgcr8, microRNAs, zebrafish, zygotic dgcr8
MeSH Terms
none
PubMed
32296464 Full text @ Front Genet
Abstract
Dgcr8 is involved in the biogenesis of canonical miRNAs to process pri-miRNA into pre-miRNA. Previous studies have provided evidence that Dgcr8 plays an essential role in different biological processes. However, the function of maternal and zygotic Dgcr8 in early embryonic development remains largely unknown. Recently, we have reported a novel approach for generating germline-specific deletions in zebrafish. This germline knockout model offers an opportunity to investigate into the differential roles of maternal or zygotic Dgcr8. Although germline specific dgcr8 deletion has no influence on gonad development, maternal or zygotic dgcr8 is essential for embryonic development in the offspring. Both maternal dgcr8 (Mdgcr8) and maternal zygotic dgcr8 (MZdgcr8) mutants display multiple developmental defects and die within 1 week. Moreover, MZdcgr8 mutant displays more severe morphogenesis defects. However, when a miR-430 duplex (the most abundantly expressed miRNA in early embryonic stage) is used to rescue the maternal mutant phenotype, the Mdgcr8 embryos could be rescued successfully and grow into adulthood and achieve sexual maturation, whereas the MZdgcr8 embryos are only partially rescued and they all die within 1 week. The differential phenotypes between the Mdgcr8 and MZdgcr8 embryos provide us with an opportunity to study the roles of individual miRNAs during early development.
Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditions5-9 somites
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-5
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-5
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-5
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-5
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-15
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsPrim-15
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsLong-pec
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsLong-pec
dgcr8zf1064/+; ihb7Tg; zf1065Tgstandard conditionsLong-pec
1 - 10 of 107
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ihb7TgTransgenic Insertion
    zf1064
      		Indel
      zf1065TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        dgcr8zf1064/+; ihb7Tg; zf1065Tg
        dgcr8zf1064/zf1064; ihb7Tg/+; zf1065Tg/+
        dgcr8zf1064/zf1064; ihb7Tg; zf1065Tg
        WT
        1 - 4 of 4
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        CreEFGCre
        EGFPEFGEGFP
        1 - 2 of 2
        Show
        Mapping
        No data available
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Zhu, Z., Liu, Y., Xu, W., Liu, T., Xie, Y., Sham, K.W.Y., Sha, O., Cheng, C.H.K. (2020) Functional Characterization and Expression Analyses Show Differential Roles of Maternal and Zygotic Dgcr8 in Early Embryonic Development. Frontiers in genetics. 11:299.