PUBLICATION

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

Authors
Gong, J., Hu, S., Huang, Z., Hu, Y., Wang, X., Zhao, J., Qian, P., Wang, C., Sheng, J., Lu, X., Wei, G., Liu, D.
ID
ZDB-PUB-200422-124
Date
2020
Source
Frontiers in molecular neuroscience   13: 34 (Journal)
Registered Authors
Gong, Jie, Liu, Dong
Keywords
Sox2, axons, development, differentiation, motor neuron, zebrafish
MeSH Terms
none
PubMed
32292330 Full text @ Front. Mol. Neurosci.
Abstract
Sex-determining region Y box 2 (Sox2), expressed in neural tissues, plays an important role as a transcription factor not only in the pluripotency and proliferation of neuronal cells but also in the opposite function of cell differentiation. Nevertheless, how Sox2 is linked to motor neuron development remains unknown. Here, we showed that Sox2 was localized in the motor neurons of spinal cord by in situ hybridization and cell separation, which acted as a positive regulator of motor neuron development. The deficiency of Sox2 in zebrafish larvae resulted in abnormal PMN development, including truncated but excessively branched CaP axons, loss of MiP, and increase of undifferentiated neuron cells. Importantly, transcriptome analysis showed that Sox2-depleted embryos caused many neurogenesis, axonogenesis, axon guidance, and differentiation-related gene expression changes, which further support the vital function of Sox2 in motor neuron development. Taken together, these data indicate that Sox2 plays a crucial role in the motor neuron development by regulating neuron differentiation and morphology of neuron axons.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ml2Tg + MO7-sox2controlPrim-5
ml2Tg + MO7-sox2controlPrim-5
ml2Tg + MO7-sox2controlLong-pec
ml2Tg + MO7-sox2controlLong-pec
ml2Tg + MO7-sox2controlLong-pec
ml2Tg + MO7-sox2controlLong-pec
ml2Tg + MO7-sox2controlProtruding-mouth
ml2Tg + MO7-sox2controlProtruding-mouth
ml2Tg + MO7-sox2controlProtruding-mouth
sox2zf3399/zf3399; ml2TgcontrolPrim-5
1 - 10 of 22
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ml2TgTransgenic Insertion
    s843TgTransgenic Insertion
      zf3399
        		Small Deletion
        1 - 3 of 3
        Show
        Human Disease / Model
        Human Disease Fish Conditions Evidence
        motor neuron diseaseTAS
        1 - 1 of 1
        Show
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        sox2MO7-sox2MRPHLNO
        sox2TALEN3-sox2TALEN
        1 - 2 of 2
        Show
        Fish
        Fish
        ml2Tg
        ml2Tg + MO7-sox2
        s843Tg
        sox2zf3399/zf3399; ml2Tg
        WT
        1 - 5 of 5
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
        Show
        Mapping
        No data available
        Your Input Welcome
        We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
        Citation
        Gong, J., Hu, S., Huang, Z., Hu, Y., Wang, X., Zhao, J., Qian, P., Wang, C., Sheng, J., Lu, X., Wei, G., Liu, D. (2020) The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience. 13:34.