PUBLICATION

Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood

Authors
Schwarzer, S., Asokan, N., Bludau, O., Kuscha, V., Kaslin, J., Hans, S.
ID
ZDB-PUB-200403-54
Date
2020
Source
Development (Cambridge, England)   147(7): (Journal)
Registered Authors
Hans, Stefan, Kaslin, Jan, Kuscha, Veronika
Keywords
Inner ear, Neuronal stem cells, PNS, Zebrafish
MeSH Terms
  • Hair Cells, Auditory/metabolism
  • Hair Cells, Auditory/physiology*
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Sensory Receptor Cells/cytology
  • Sensory Receptor Cells/physiology
  • Animals
  • Aging/physiology
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
  • Zebrafish*/metabolism
  • Gene Expression Regulation, Developmental
  • Cell Differentiation/genetics
  • Adult Stem Cells/cytology
  • Adult Stem Cells/physiology*
  • Nestin/metabolism
  • Larva
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Neural Stem Cells/physiology*
  • Ear, Inner/cytology
  • Ear, Inner/physiology*
  • Stem Cell Niche/physiology
  • Neurogenesis/physiology*
  • Ganglia, Sensory/cytology*
  • Ganglia, Sensory/physiology
  • Embryo, Nonmammalian
  • Nerve Tissue Proteins/metabolism
  • Animals, Genetically Modified
(all 29)
PubMed
32165493 Full text @ Development
Abstract
The vertebrate inner ear employs sensory hair cells and neurons to mediate hearing and balance. In mammals, damaged hair cells and neurons are not regenerated. In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. However, it is unknown whether new sensory neurons are also formed in the adult zebrafish statoacoustic ganglion (SAG), the sensory ganglion connecting the inner ear to the brain. Using transgenic lines and marker analysis, we identify distinct cell populations and anatomical landmarks in the juvenile and adult SAG. In particular, we analyze a Neurod/Nestin-positive progenitor pool that produces large amounts of new neurons at juvenile stages, which transitions to a quiescent state in the adult SAG. Moreover, BrdU pulse chase experiments reveal the existence of a proliferative but otherwise marker-negative cell population that replenishes the Neurod/Nestin-positive progenitor pool at adult stages. Taken together, our study represents the first comprehensive characterization of the adult zebrafish SAG showing that zebrafish, in sharp contrast to mammals, display continued neurogenesis in the SAG well beyond embryonic and larval stages.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nl1TgTransgenic Insertion
    s356tTgTransgenic Insertion
      stl84TgTransgenic Insertion
        tud9TgTransgenic Insertion
          tud45TgTransgenic Insertion
            tud100TgTransgenic Insertion
              tud103TgTransgenic Insertion
                y83TgTransgenic Insertion
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  No data available
                  Antibodies
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  CreEFGCre
                  DsRed2EFGDsRed2
                  EGFPEFGEGFP
                  GFPEFGGFP
                  mCherryEFGmCherry
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                  Mapping
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                  Citation
                  Schwarzer, S., Asokan, N., Bludau, O., Kuscha, V., Kaslin, J., Hans, S. (2020) Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood. Development (Cambridge, England). 147(7):.