PUBLICATION

Mitochondria in Embryogenesis: An Organellogenesis Perspective

Authors
Arribat, Y., Grepper, D., Lagarrigue, S., Richard, J., Gachet, M., Gut, P., Amati, F.
ID
ZDB-PUB-191212-37
Date
2019
Source
Frontiers in cell and developmental biology   7: 282 (Journal)
Registered Authors
Amati, Francesca, Arribat, Yoan, Grepper, Dogan, Gut, Philipp, Lagarrigue, Silviane, Richard, Joy
Keywords
electron transport chain supercomplexes, mitochondria fission, morphogenes, myomaker, somite, sonic hedgehog, zebrafish
MeSH Terms
none
PubMed
31824944 Full text @ Front Cell Dev Biol
Abstract
Organogenesis is well characterized in vertebrates. However, the anatomical and functional development of intracellular compartments during this phase of development remains unknown. Taking an organellogenesis point of view, we characterize the spatiotemporal adaptations of the mitochondrial network during zebrafish embryogenesis. Using state of the art microscopy approaches, we find that mitochondrial network follows three distinct distribution patterns during embryonic development. Despite of this constant morphological change of the mitochondrial network, electron transport chain supercomplexes occur at early stages of embryonic development and conserve a stable organization throughout development. The remodeling of the mitochondrial network and the conservation of its structural components go hand-in-hand with somite maturation; for example, genetic disruption of myoblast fusion impairs mitochondrial network maturation. Reciprocally, mitochondria quality represents a key factor to determine embryonic progression. Alteration of mitochondrial polarization and electron transport chain halts embryonic development in a reversible manner suggesting developmental checkpoints that depend on mitochondrial integrity. Our findings establish the subtle dialogue and co-dependence between organogenesis and mitochondria in early vertebrate development. They also suggest the importance of adopting subcellular perspectives to understand organelle-organ communications during embryogenesis.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
bnip3lbABstandard conditionsAdultRTPCR
bnip3lbABstandard conditionsPrim-5RTPCR
bnip3lbABstandard conditionsLong-pecRTPCR
bnip3lbABstandard conditionsPrim-5RTPCR
bnip3lbABstandard conditions4-cellRTPCR
bnip3lbABstandard conditionsPrim-25RTPCR
bnip3lbABstandard conditions8-cellRTPCR
bnip3lbABstandard conditionsLong-pecRTPCR
bnip3lbABstandard conditions14-19 somitesRTPCR
dnm1aABstandard conditionsAdultRTPCR
1 - 10 of 88
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Phenotype
Fish Conditions Stage Phenotype Figure
nei007Tg (AB)chemical treatment by environment: CyclopaminePrim-5
nei007Tg (AB)chemical treatment by environment: CyclopamineLong-pec
nei007Tg (AB)chemical treatment by environment: CyclopamineLong-pec
nei007Tg (AB)chemical treatment by environment: DMH1Prim-5
nei007Tg (AB)chemical treatment by environment: DMH1Long-pec
nei007Tg (AB)chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazonePrim-5 to Long-pec
nei007Tg (AB)chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazoneLong-pec
nei007Tg (AB)chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazoneLong-pec
nei007Tg (AB)chemical treatment by environment: carbonyl cyanide p-trifluoromethoxyphenylhydrazoneDay 5
nei007Tg (AB)chemical treatment by environment: quinazolinesPrim-5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nei007TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    mymkMO1-mymkMRPHLNO
    mymkMO2-mymkMRPHLNO
    1 - 2 of 2
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    Fish
    Fish
    AB
    nei007Tg (AB)
    nei007Tg + MO1-mymk (AB)
    nei007Tg + MO2-mymk (AB)
    1 - 4 of 4
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    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    TomatoEFGTomato
    ZsGreen1EFGZsGreen1
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    Mapping
    No data available
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    Citation
    Arribat, Y., Grepper, D., Lagarrigue, S., Richard, J., Gachet, M., Gut, P., Amati, F. (2019) Mitochondria in Embryogenesis: An Organellogenesis Perspective. Frontiers in cell and developmental biology. 7:282.