PUBLICATION

Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells

Authors
Chestnut, B., Sumanas, S.
ID
ZDB-PUB-191111-9
Date
2019
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   249(2): 245-261 (Journal)
Registered Authors
Sumanas, Saulius
Keywords
CRISPR, Cas9, RNA-seq, myeloid, red blood cell, transcriptome, vascular endothelial, zebrafish
Datasets
GEO:GSE139108
MeSH Terms
  • Animals
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Embryo, Nonmammalian/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Clustered Regularly Interspaced Short Palindromic Repeats/genetics
  • CRISPR-Associated Protein 9/genetics
  • CRISPR-Associated Protein 9/metabolism
  • Transcriptome/genetics
(all 14)
PubMed
31705559 Full text @ Dev. Dyn.
Abstract
ETS transcription factor Etv2 / Etsrp is one of the earliest markers for vascular and hematopoietic progenitors and functions as a key regulator of hematovascular development in multiple vertebrates, including zebrafish. Therefore transgenic etv2 reporter lines provide a valuable tool to study vasculogenesis and hematopoiesis. However, previously generated zebrafish reporter lines do not fully recapitulate the endogenous pattern of etv2 expression.
Here we used CRISPR / Cas9-mediated homology-independent DNA repair approach to knock-in a Gal4 transcriptional activator into the zebrafish etv2 genomic locus, thus generating etv2ci32Gt gene trap line. etv2ci32Gt ; UAS:GFP embryos show GFP expression in vascular endothelial, myeloid and red blood cells. Because gal4 insertion interrupts the etv2 locus, homozygous etv2ci32Gt embryos display defects in vasculogenesis and myelopoiesis, and enable visualizing etv2-deficient hematovascular progenitors in live embryos. Furthermore, we performed differential transcriptome analysis of sorted GFP-positive cells from heterozygous and homozygous etv2ci32Gt embryos. Approximately 500 downregulated genes were identified in etv2ci32Gt homozygous embryos, which include multiple genes expressed in vascular endothelial and myeloid cells.
The etv2ci32Gt gene trap line and the datasets of misregulated genes will be valuable resources to study hematopoietic and vascular development. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci1TgTransgenic Insertion
    ci5TgTransgenic Insertion
      ci32GtTransgenic Insertion
      nkuasgfp1aTgTransgenic Insertion
        zf372TgTransgenic Insertion
          1 - 5 of 5
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          etsrpCRISPR2-etsrpCRISPR
          1 - 1 of 1
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          Fish
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GAL4EFGGAL4
          mCherryEFGmCherry
          1 - 3 of 3
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          Mapping
          No data available