PUBLICATION

A bidirectional network for appetite control in larval zebrafish

Authors
Wee, C.L., Song, E.Y., Johnson, R.E., Ailani, D., Randlett, O., Kim, J.Y., Nikitchenko, M., Bahl, A., Yang, C.T., Ahrens, M.B., Kawakami, K., Engert, F., Kunes, S.
ID
ZDB-PUB-191022-17
Date
2019
Source
eLIFE   8: (Journal)
Registered Authors
Ahrens, Misha, Engert, Florian, Kawakami, Koichi, Randlett, Owen, Yang, Chao-Tsung
Keywords
appetite, hypothalamus, neuroscience, serotonin, zebrafish
MeSH Terms
  • Hypothalamus/physiology*
  • Zebrafish/physiology*
  • Appetite*
  • Larva/physiology
  • Serotonergic Neurons/physiology*
  • Nerve Net/physiology*
  • Animals
(all 7)
PubMed
31625906 Full text @ Elife
Abstract
Medial and lateral hypothalamic loci are known to suppress and enhance appetite, respectively, but the dynamics and functional significance of their interaction have yet to be explored. Here we report that, in larval zebrafish, primarily serotonergic neurons of the ventromedial caudal hypothalamus (cH) become increasingly active during food deprivation, whereas activity in the lateral hypothalamus (LH) is reduced. Exposure to food sensory and consummatory cues reverses the activity patterns of these two nuclei, consistent with their representation of opposing internal hunger states. Baseline activity is restored as food-deprived animals return to satiety via voracious feeding. The antagonistic relationship and functional importance of cH and LH activity patterns were confirmed by targeted stimulation and ablation of cH neurons. Collectively, the data allow us to propose a model in which these hypothalamic nuclei regulate different phases of hunger and satiety and coordinate energy balance via antagonistic control of distinct behavioral outputs.
Genes / Markers
Figures
Figure Gallery (17 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a13203TgTransgenic Insertion
    c264TgTransgenic Insertion
      ct819TgTransgenic Insertion
        jf50TgTransgenic Insertion
          nkgsaizGFFM116AGtTransgenic Insertion
            nkhspGFFDMC76AEtTransgenic Insertion
              nkUAShspzGCaMP6s13aTgTransgenic Insertion
                nns9TgTransgenic Insertion
                  nns25TgTransgenic Insertion
                    nns26TgTransgenic Insertion
                      1 - 10 of 13
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GAL4FFEFGGAL4FF
                      GCaMPEFGGCaMP
                      GFPEFGGFP
                      mCherryEFGmCherry
                      NTREFGNTR
                      ReaChREFGReaChR
                      RFPEFGRFP
                      TagRFPTEFGTagRFPT
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                      Citation
                      Wee, C.L., Song, E.Y., Johnson, R.E., Ailani, D., Randlett, O., Kim, J.Y., Nikitchenko, M., Bahl, A., Yang, C.T., Ahrens, M.B., Kawakami, K., Engert, F., Kunes, S. (2019) A bidirectional network for appetite control in larval zebrafish. eLIFE. 8:.