PUBLICATION

Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts

Authors
Peng, L., Zhou, Y., Xu, W., Jiang, M., Li, H., Long, M., Liu, W., Liu, J., Zhao, X., Xiao, Y.
ID
ZDB-PUB-191011-14
Date
2019
Source
International journal of biological sciences   15: 2340-2349 (Journal)
Registered Authors
Keywords
doxycycline, fibroblast, induced pluripotent stem cell, lentiviral delivery system, zebra fish
MeSH Terms
  • Cell Differentiation
  • Induced Pluripotent Stem Cells/cytology*
  • Cell Line
  • Fibroblasts/cytology*
  • Transduction, Genetic
  • Zebrafish/embryology
  • Animals
  • Doxycycline
(all 8)
PubMed
31595152 Full text @ Int. J. Biol. Sci.
Abstract
Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established for mammals, few attempts have been made to induce iPS cells in nonmammalian species. Because of technical advantages over other vertebrates on stem cells, induced pluripotent stem cells from fish could be of value for research. In this paper, stable iPS-like cell lines were generated from adult zebra fish fibroblasts by combining the doxycycline inducible lentiviral delivery system and chemical treatment. RT-PCR analysis, alkaline phosphatase staining, and immunofluorescence indicated that adult zebra fish fibroblasts were successfully reprogrammed into iPS-like cells (ziPSCs). The ziPSCs exhibited stable growth and manifested many features of fish embryonic stem cells with pluripotency in vitro and in vivo. Because of easy maintenance, the developed technology in this study for generating zebra fish iPS-like cells could be extended to investigating other genera of fish.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab4-nespolyclonal
    		IgGRabbit
    Ab13-sox2monoclonalIgG1Mouse
    1 - 2 of 2
    Show
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Peng, L., Zhou, Y., Xu, W., Jiang, M., Li, H., Long, M., Liu, W., Liu, J., Zhao, X., Xiao, Y. (2019) Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts. International journal of biological sciences. 15:2340-2349.