PUBLICATION

Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit

Authors
Wan, Y., Wei, Z., Looger, L.L., Koyama, M., Druckmann, S., Keller, P.J.
ID
ZDB-PUB-191001-2
Date
2019
Source
Cell   179(2): 355-372.e23 (Journal)
Registered Authors
Keller, Philipp, Wan, Yinan
Keywords
calcium imaging, circuit development, computational data analysis, embryonic development, light-sheet microscopy, population activity, spinal cord, zebrafish
MeSH Terms
none
PubMed
31564455 Full text @ Cell
Abstract
Animal survival requires a functioning nervous system to develop during embryogenesis. Newborn neurons must assemble into circuits producing activity patterns capable of instructing behaviors. Elucidating how this process is coordinated requires new methods that follow maturation and activity of all cells across a developing circuit. We present an imaging method for comprehensively tracking neuron lineages, movements, molecular identities, and activity in the entire developing zebrafish spinal cord, from neurogenesis until the emergence of patterned activity instructing the earliest spontaneous motor behavior. We found that motoneurons are active first and form local patterned ensembles with neighboring neurons. These ensembles merge, synchronize globally after reaching a threshold size, and finally recruit commissural interneurons to orchestrate the left-right alternating patterns important for locomotion in vertebrates. Individual neurons undergo functional maturation stereotypically based on their birth time and anatomical origin. Our study provides a general strategy for reconstructing how functioning circuits emerge during embryogenesis. VIDEO ABSTRACT.
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Allele Construct Type Affected Genomic Region
jf1TgTransgenic Insertion
    jf7TgTransgenic Insertion
      nns44TgTransgenic Insertion
        s1020tEtTransgenic Insertion
          ta229g
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            vu504TgTransgenic Insertion
              zf3217TgTransgenic Insertion
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                GAL4EFGGAL4
                GCaMPEFGGCaMP
                HaloTagEFGHaloTag
                RFPEFGRFP
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                YFPEFGYFP
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                Citation
                Wan, Y., Wei, Z., Looger, L.L., Koyama, M., Druckmann, S., Keller, P.J. (2019) Single-Cell Reconstruction of Emerging Population Activity in an Entire Developing Circuit. Cell. 179(2):355-372.e23.