PUBLICATION

Zebrafish prmt5 arginine methyltransferase is essential for germ cell development

Authors
Zhu, J., Zhang, D., Liu, X., Yu, G., Cai, X., Xu, C., Rong, F., Ouyang, G., Wang, J., Xiao, W.
ID
ZDB-PUB-190920-3
Date
2019
Source
Development (Cambridge, England)   146(20): (Journal)
Registered Authors
Ouyang, Gang, Wang, Jing, Xiao, Wuhan
Keywords
Germ cell, Vasa, Zebrafish, Zili, Ziwi, prmt5
MeSH Terms
  • Meiosis/physiology
  • Zebrafish
  • Methylation
  • Protein-Arginine N-Methyltransferases/genetics
  • Protein-Arginine N-Methyltransferases/metabolism*
  • Animals
  • Phenotype
  • Histones/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Gonads/cytology
  • Gonads/metabolism
  • Ovary/cytology
  • Ovary/metabolism
  • Cell Movement/genetics
  • Cell Movement/physiology
  • Male
  • Arginine/metabolism
  • Infertility, Male/metabolism
  • Germ Cells/metabolism*
  • Female
  • Testis/cytology
  • Testis/metabolism
(all 23)
PubMed
31533925 Full text @ Development
Abstract
Protein arginine methyltransferase 5 (Prmt5), a type II arginine methyltransferase, symmetrically dimethylates arginine in nuclear and cytoplasmic proteins. Prmt5 is involved in a variety of cellular processes including ribosome biogenesis, cellular differentiation, germ cell development, and tumorigenesis. However, the mechanisms by which prmt5 influences cellular processes have remained unclear. Here, prmt5 loss in zebrafish led to the expression of an infertile male phenotype due to a reduction in germ cell number, an increase in germ cell apoptosis, and the failure of gonads to differentiate into normal testes or ovaries. Moreover, arginine methylation of the germ-cell-specific proteins Zili and Vasa, as well as histones H3 (H3R8me2s) and H4 (H4R3me2s), was reduced in the gonads of prmt5-null zebrafish. This resulted in the downregulation of several Piwi pathway proteins, including Zili, and Vasa. In addition, various genes related to meiosis, gonad development, and sexual differentiation were dysregulated in the gonads of prmt5-null zebrafish. Our results revealed a novel mechanism associated with prmt5, i.e., prmt5 apparently controls germ cell development in vertebrates by catalyzing arginine methylation of the germline-specific proteins Zili and Vasa.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
prmt5ihb1994/+ (AB)standard conditionsAdult
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 30-44
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 45-89
prmt5ihb1994/+; prmt5ihb1995/+ (AB)standard conditionsDays 45-89
1 - 10 of 82
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ihb1994
    		Small Deletion
    ihb1995
      		Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      AB
      prmt5ihb1994/ihb1994 (AB)
      prmt5ihb1994/+ (AB)
      prmt5ihb1994/+; prmt5ihb1995/+ (AB)
      prmt5ihb1995/ihb1995 (AB)
      1 - 5 of 5
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      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-ASYM24polyclonal
        		IgGRabbit
        Ab1-sDMApolyclonal
          		IgGRabbit
          AB2-piwil2polyclonalRabbit
          AB2-prmt5monoclonalRabbit
          Ab4-ddx4polyclonalIgGRabbit
          AB4-piwil1polyclonalRabbit
          1 - 6 of 6
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          Orthology
          Gene Orthology
          prmt5
          1 - 1 of 1
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          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Zhu, J., Zhang, D., Liu, X., Yu, G., Cai, X., Xu, C., Rong, F., Ouyang, G., Wang, J., Xiao, W. (2019) Zebrafish prmt5 arginine methyltransferase is essential for germ cell development. Development (Cambridge, England). 146(20):.