PUBLICATION

Stochastic cell-cycle entry and cell-state-dependent fate outputs of injury-reactivated tectal radial glia in zebrafish

Authors
Yu, S., He, J.
ID
ZDB-PUB-190826-8
Date
2019
Source
eLIFE   8: (Journal)
Registered Authors
He, Jie, Yu, Shuguang
Keywords
Notch, developmental biology, gliosis, injury, radial glia, reactivation, regenerative medicine, stem cells, the optic tectum, zebrafish
MeSH Terms
  • Disease Models, Animal
  • Animals
  • Cell Cycle*
  • Neuroglia/physiology*
  • Wounds and Injuries/complications*
  • Cell Proliferation
  • Gliosis/physiopathology*
  • Superior Colliculi/injuries*
  • Zebrafish
(all 9)
PubMed
31442201 Full text @ Elife
Abstract
Gliosis defined as reactive changes of resident glia is the primary response of the central nervous system (CNS) to trauma. The proliferation and fate controls of injury-reactivated glia are essential but remain largely unexplored. In zebrafish optic tectum, we found that stab injury drove a subset of radial glia (RG) into the cell cycle, and surprisingly, proliferative RG responding to sequential injuries of the same site were distinct but overlapping, which was in agreement with stochastic cell-cycle entry. Single-cell RNA sequencing analysis and functional assays further revealed the involvement of Notch/Delta lateral inhibition in this stochastic cell-cycle entry. Furthermore, the long-term clonal analysis showed that proliferative RG were largely gliogenic. Notch inhibition of reactive RG, not dormant and proliferative RG, resulted in an increased production of neurons, which were short-lived. Our findings gain new insights into the proliferation and fate controls of injury-reactivated CNS glia in zebrafish.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ion4hTgTransgenic Insertion
    ion5hTgTransgenic Insertion
      ion6hTgTransgenic Insertion
        ka101TgTransgenic Insertion
          kca3TgTransgenic Insertion
            kca4TgTransgenic Insertion
              knu2TgTransgenic Insertion
                mi4TgTransgenic Insertion
                  mi2001TgTransgenic Insertion
                    vu12TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      RFPEFGRFP
                      1 - 7 of 7
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                      Mapping
                      No data available
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                      Citation
                      Yu, S., He, J. (2019) Stochastic cell-cycle entry and cell-state-dependent fate outputs of injury-reactivated tectal radial glia in zebrafish. eLIFE. 8.