PUBLICATION

Bacteria evoke alarm behaviour in zebrafish

Authors
Chia, J.S.M., Wall, E.S., Wee, C.L., Rowland, T.A.J., Cheng, R.K., Cheow, K., Guillemin, K., Jesuthasan, S.
ID
ZDB-PUB-190826-15
Date
2019
Source
Nature communications   10: 3831 (Journal)
Registered Authors
Guillemin, Karen, Jesuthasan, Suresh
Keywords
none
MeSH Terms
  • Animal Communication*
  • Symbiosis/physiology
  • Mucus/cytology
  • Mucus/metabolism
  • Mucus/microbiology
  • Intravital Microscopy
  • Skin/cytology
  • Skin/metabolism*
  • Skin/microbiology
  • Giant Cells/metabolism
  • Giant Cells/microbiology
  • Fear/physiology
  • Animals, Genetically Modified
  • Zebrafish/injuries
  • Zebrafish/microbiology
  • Zebrafish/physiology*
  • Staphylococcus/metabolism*
  • Animals
  • Neutrophils/metabolism
  • Neutrophils/microbiology
  • Reflex, Startle/physiology
  • Optical Imaging
(all 22)
PubMed
31444339 Full text @ Nat. Commun.
Abstract
When injured, fish release an alarm substance (Schreckstoff) that elicits fear in members of their shoal. Although Schreckstoff has been proposed to be produced by club cells in the skin, several observations indicate that these giant cells function primarily in immunity. Previous data indicate that the alarm substance can be isolated from mucus. Here we show that mucus, as well as bacteria, are transported from the external surface into club cells, by cytoplasmic transfer or invasion of cells, including neutrophils. The presence of bacteria inside club cells raises the possibility that the alarm substance may contain a bacterial component. Indeed, lysate from a zebrafish Staphylococcus isolate is sufficient to elicit alarm behaviour, acting in concert with a substance from fish. These results suggest that Schreckstoff, which allows one individual to unwittingly change the emotional state of the surrounding population, derives from two kingdoms and is associated with processes that protect the host from bacteria.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c426TgTransgenic Insertion
    e114TgTransgenic Insertion
      p209TgTransgenic Insertion
        rw0320TgTransgenic Insertion
          sq205TgTransgenic Insertion
            sq214TgTransgenic Insertion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              No data available
              Fish
              No data available
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              Ab2-ctnnbmonoclonalIgG1Mouse
              Ab5-casp3polyclonal
                		Rabbit
                1 - 2 of 2
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                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                GAL4EFGGAL4
                GAL4FFEFGGAL4FF
                GCaMPEFGGCaMP
                GFPEFGGFP
                1 - 4 of 4
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                Mapping
                No data available
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                Citation
                Chia, J.S.M., Wall, E.S., Wee, C.L., Rowland, T.A.J., Cheng, R.K., Cheow, K., Guillemin, K., Jesuthasan, S. (2019) Bacteria evoke alarm behaviour in zebrafish. Nature communications. 10:3831.