PUBLICATION

Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation

Authors

Jiang, D., Jiang, Z., Lu, D., Wang, X., Liang, H., Zhang, J., Meng, Y., Li, Y., Wu, D., Huang, Y., Chen, Y., Deng, H., Wu, Q., Xiong, J., Meng, A., Yu, L.

ID

ZDB-PUB-190803-9

Date

2019

Source

Nature cell biology 21: 966-977 (Journal)

Registered Authors

Li, Ying, Meng, Anming, Xiong, Jing-Wei, Zhang, Junfeng

Keywords

none

MeSH Terms

Body Patterning/physiology
Morphogenesis/physiology*
Zebrafish/embryology
Animals
Embryonic Development/physiology
Signal Transduction/genetics
Signal Transduction/physiology
Zebrafish Proteins/genetics*
Cell Movement/genetics
Cell Movement/physiology
Organelles/genetics
Organelles/metabolism*
Embryo, Nonmammalian/metabolism*
Gastrulation/physiology

(all 14)

PubMed

31371827 Full text @ Nat. Cell Biol.

Abstract

Migrasomes are recently identified vesicular organelles that form on retraction fibres behind migrating cells. Whether migrasomes are present in vivo and, if so, the function of migrasomes in living organisms is unknown. Here, we show that migrasomes are formed during zebrafish gastrulation and signalling molecules, such as chemokines, are enriched in migrasomes. We further demonstrate that Tspan4 and Tspan7 are required for migrasome formation. Organ morphogenesis is impaired in zebrafish MZtspan4a and MZtspan7 mutants. Mechanistically, migrasomes are enriched on a cavity underneath the embryonic shield where they serve as chemoattractants to ensure the correct positioning of dorsal forerunner cells vegetally next to the embryonic shield, thereby affecting organ morphogenesis. Our study shows that migrasomes are signalling organelles that provide specific biochemical information to coordinate organ morphogenesis.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cp	tspan4a^{tsu2cd/tsu2cd}	standard conditions	Long-pec	liver	ISH	Fig. 3
cp	tspan6^{tsu10ci/tsu10ci}	standard conditions	Long-pec	liver	ISH	Fig. 3
cp	TU	standard conditions	Long-pec	liver	ISH	Fig. 3
cxcl12a	TU	standard conditions	Shield to 90%-epiboly	whole organism migrasome	IHC	Fig. 4
cxcl12a	TU	standard conditions	Shield to 90%-epiboly	whole organism migrasome	WB	Fig. 4
cxcr4a	TU	standard conditions	Shield to 90%-epiboly	forerunner cell group	ISH	Fig. S7
cxcr4b	s870Tg	standard conditions	Shield	forerunner cell group	IHC	Fig. 7
foxa3	tspan4a^{tsu2cd/tsu2cd}	standard conditions	Long-pec	intestinal bulb	ISH	Fig. 3
foxa3	tspan6^{tsu10ci/tsu10ci}	standard conditions	Long-pec	intestinal bulb	ISH	Fig. 3
foxa3	TU	standard conditions	Long-pec	intestinal bulb	ISH	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
TU + MO1-tspan4a	control	Shield	whole organism migrasome decreased amount, abnormal	Fig. S2
TU + MO1-tspan6	control	Shield	whole organism migrasome decreased amount, abnormal	Fig. S2
cxcl12a^{tsu4cd/tsu4cd}	standard conditions	Shield to 90%-epiboly	determination of left/right symmetry decreased occurrence, abnormal	Fig. S5
cxcr4b^{ioz106/ioz106}	standard conditions	Shield to 90%-epiboly	determination of left/right symmetry decreased occurrence, abnormal	Fig. S5
cxcr4b^{ioz106/ioz106}	standard conditions	Shield to 90%-epiboly	forerunner cell group sox17 expression spatial pattern, abnormal	Fig. S7
cxcr4b^{ioz106/ioz106}	standard conditions	Shield to 90%-epiboly	forerunner cell group distributed, abnormal	Fig. S7
cxcr4b^{ioz106/ioz106}	standard conditions	Shield to 90%-epiboly	forerunner cell group irregular spatial pattern, abnormal	Fig. S7
itgb1b^{pku370/pku370}	control	Shield	whole organism migrasome decreased amount, abnormal	Fig. S2
tspan4a^{tsu2cd/tsu2cd}	control	Shield to 90%-epiboly	cell migration involved in gastrulation process quality, normal	Fig. 2
tspan4a^{tsu2cd/tsu2cd}	control	Shield to 90%-epiboly	cell migration involved in gastrulation process quality, normal	Fig. S3

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ioz106		Small Deletion	cxcr4b
pku370		Indel	itgb1b
s870Tg	Tg(sox17:GFP)	Transgenic Insertion
tsu2cd		Small Deletion	tspan4a
tsu4cd		Small Deletion	cxcl12a
tsu10ci		Insertion	tspan6

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
cxcl12a	CRISPR2-cxcl12a	CRISPR
cxcl12a	MO2-cxcl12a	MRPHLNO
cxcl12b	MO1-cxcl12b	MRPHLNO
cxcr4b	CRISPR2-cxcr4b	CRISPR
itgb1b	MO2-itgb1b	MRPHLNO
itgb1b	TALEN1-itgb1b	TALEN
tspan4a	CRISPR1-tspan4a	CRISPR
tspan4a	MO1-tspan4a	MRPHLNO
tspan6	CRISPR1-tspan6	CRISPR
tspan6	MO1-tspan6	MRPHLNO

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Fish

Fish
cxcl12a^{tsu4cd/tsu4cd}
cxcr4b^{ioz106/ioz106}
itgb1b^{pku370/pku370}
s870Tg
tspan4a^{tsu2cd/tsu2cd}
tspan4a^{tsu2cd/tsu2cd}; s870Tg
tspan6^{tsu10ci/tsu10ci}
tspan6^{tsu10ci/tsu10ci}; s870Tg
TU
TU + MO1-tspan4a

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Jiang et al., 2019 ZDB-PUB-190803-9 PMID:31371827

Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation

Jiang et al., 2019

ZDB-PUB-190803-9
PMID:31371827