PUBLICATION

Distinct inflammatory and wound healing responses to complex caudal fin injuries of larval zebrafish

Authors

Miskolci, V., Squirrell, J., Rindy, J., Vincent, W., Sauer, J.D., Gibson, A., Eliceiri, K.W., Huttenlocher, A.

ID

ZDB-PUB-190702-4

Date

2019

Source

eLIFE 8: (Journal)

Registered Authors

Huttenlocher, Anna

Keywords

cell biology, infection, inflammation, thermal injury, wound healing, zebrafish

MeSH Terms

Wound Healing*
Inflammation/pathology*
Inflammation/physiopathology*
Zebrafish
Animals
Wounds and Injuries/pathology*

(all 6)

PubMed

31259685 Full text @ Elife

Abstract

Wound repair is controlled temporally and spatially to restore tissue homeostasis. Previously we reported that thermal damage of the larval zebrafish fin disrupts collagen organization and wound healing compared to tail transection (LeBert et al., 2018). Here we characterize different injury models in larval zebrafish to dissect temporal and spatial dynamics of repair in complex damage. We found that each damage model triggers distinct inflammatory and tissue responses, with Stat3 and TGFβ playing key roles in the regulation of mesenchymal cells during simple repair. While thermal injury disrupts collagen fibers initially, healing is recovered as inflammation resolves, and mesenchymal cells and collagen fibers align. By contrast, infected wounds lead to persistent inflammation and loss of mesenchymal cells, resulting in minimal tissue repair. These wound models have broad physiological relevance, thereby providing a valuable advance in our toolkit to probe the dynamics of inflammation and wound repair in complex tissue damage.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB	bacterial treatment by exposure to environment: Listeria monocytogenes 10403S, amputation: caudal fin	Protruding-mouth to Days 7-13	fin regeneration decreased efficacy, abnormal	Fig. 1
AB	bacterial treatment by exposure to environment: Listeria monocytogenes 10403S, amputation: caudal fin	Protruding-mouth to Days 7-13	regenerating fin collagen network decreased amount, abnormal	Fig. 3
AB	bacterial treatment by exposure to environment: Listeria monocytogenes 10403S, amputation: caudal fin	Protruding-mouth to Days 7-13	regenerating fin collagen network morphology, abnormal	Fig. 3
AB	thermal cauterization: regenerating fin, amputation: caudal fin	Protruding-mouth to Days 7-13	fin regeneration decreased rate, abnormal	Fig. 1
AB	thermal cauterization: regenerating fin, amputation: caudal fin	Protruding-mouth to Days 7-13	regenerating fin collagen network decreased amount, abnormal	Fig. 3
pd1028Tg; sh378Tg; zf2171Tg	bacterial treatment by exposure to environment: Listeria monocytogenes 10403S, amputation: caudal fin	Protruding-mouth to Days 7-13	macrophage chemotaxis increased occurrence, abnormal	Fig. 2
pd1028Tg; sh378Tg; zf2171Tg	bacterial treatment by exposure to environment: Listeria monocytogenes 10403S, amputation: caudal fin	Protruding-mouth to Days 7-13	neutrophil chemotaxis increased occurrence, abnormal	Fig. 2
pd1028Tg; sh378Tg; zf2171Tg	thermal cauterization: regenerating fin, amputation: caudal fin	Protruding-mouth to Days 7-13	macrophage chemotaxis increased occurrence, abnormal	Fig. 2
pd1028Tg; sh378Tg; zf2171Tg	thermal cauterization: regenerating fin, amputation: caudal fin	Protruding-mouth to Days 7-13	neutrophil chemotaxis increased occurrence, abnormal	Fig. 2
stat3^stl27/stl27	amputation: caudal fin	Day 6	fin regeneration decreased efficacy, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
a9		Complex	mpv17
pd1028Tg	TgBAC(tnfa:GFP)	Transgenic Insertion
sh378Tg	Tg(mpeg1.1:mCherry-CAAX)	Transgenic Insertion
stl27		Small Deletion	stat3
uwm26Tg	Tg(mpeg1.1:mCherry-Hsa.HIST1H2BJ)	Transgenic Insertion
uwm37Tg	Tg(-2vim:EGFP)	Transgenic Insertion
uwm40Tg	Tg(lyz:H2B-mCherry)	Transgenic Insertion
uwm43Tg	Tg(mpeg1.1:GFP-Hsa.HIST1H2BJ)	Transgenic Insertion
w2		Point Mutation	mitfa
zf2171Tg	Tg(lyz:BFP)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
pd1028Tg; sh378Tg; zf2171Tg
stat3^stl27/stl27
stat3^stl27/stl27; uwm37Tg
uwm37Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
BFP	EFG	BFP
EGFP	EFG	EGFP
GFP	EFG	GFP
mCherry	EFG	mCherry

Mapping

Miskolci et al., 2019 ZDB-PUB-190702-4 PMID:31259685

Distinct inflammatory and wound healing responses to complex caudal fin injuries of larval zebrafish

Miskolci et al., 2019

ZDB-PUB-190702-4
PMID:31259685