PUBLICATION

Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation

Authors
Hadjivasiliou, Z., Moore, R.E., McIntosh, R., Galea, G.L., Clarke, J.D.W., Alexandre, P.
ID
ZDB-PUB-190619-10
Date
2019
Source
Developmental Cell   49: 907-919.e10 (Journal)
Registered Authors
Alexandre, Paula, Clarke, Jon
Keywords
basal protrusions, lateral inhibition, live imaging, neuronal differentiation, spatiotemporal pattern, spinal cord, zebrafish
MeSH Terms
  • Neurogenesis
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Spinal Cord/cytology*
  • Spinal Cord/metabolism
  • Motor Neurons/cytology*
  • Motor Neurons/metabolism
  • Gene Expression Regulation, Developmental
  • Animals
  • Cell Communication
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Signal Transduction
  • Laminin/genetics
  • Laminin/metabolism*
  • Body Patterning*
  • Cell Differentiation*
  • Spatio-Temporal Analysis
(all 24)
PubMed
31211994 Full text @ Dev. Cell
Abstract
During early spinal cord development, neurons of particular subtypes differentiate with a sparse periodic pattern while later neurons differentiate in the intervening space to eventually produce continuous columns of similar neurons. The mechanisms that regulate this spatiotemporal pattern are unknown. In vivo imaging in zebrafish reveals that differentiating spinal neurons transiently extend two long protrusions along the basal surface of the spinal cord before axon initiation. These protrusions express Delta protein, consistent with the hypothesis they influence Notch signaling at a distance of several cell diameters. Experimental reduction of Laminin expression leads to smaller protrusions and shorter distances between differentiating neurons. The experimental data and a theoretical model support the proposal that neuronal differentiation pattern is regulated by transient basal protrusions that deliver temporally controlled lateral inhibition mediated at a distance. This work uncovers a stereotyped protrusive activity of newborn neurons that organize long-distance spatiotemporal patterning of differentiation.
Genes / Markers
Figures
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Expression
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca2TgTransgenic Insertion
    nkuasgfp1aTgTransgenic Insertion
      nns5TgTransgenic Insertion
        s940TgTransgenic Insertion
          sa379
            		Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab1-dldmonoclonalIgG1Mouse
            Ab1-elavlmonoclonalIgG2bMouse
            Ab1-prkczpolyclonalIgGRabbit
            1 - 3 of 3
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GAL4EFGGAL4
            GFPEFGGFP
            VenusEFGVenus
            1 - 4 of 4
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            Mapping
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            Citation
            Hadjivasiliou, Z., Moore, R.E., McIntosh, R., Galea, G.L., Clarke, J.D.W., Alexandre, P. (2019) Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation. Developmental Cell. 49:907-919.e10.