PUBLICATION

Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish CNS

Authors
Kim, E., Jeong, I., Chung, A.Y., Kim, S., Kwon, S.H., Seong, J.Y., Park, H.C.
ID
ZDB-PUB-190326-3
Date
2019
Source
Scientific Reports   9: 5025 (Journal)
Registered Authors
Chung, Ah-Young, Jeong, Inyoung, Kim, Eunmi, Kim, Suhyun, Park, Hae-Chul
Keywords
none
MeSH Terms
  • Nerve Net/cytology
  • Nerve Net/metabolism*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Animals, Genetically Modified
  • Rhombencephalon/metabolism
  • Animals
  • Gene Expression
  • Larva/genetics
  • Larva/metabolism
  • Mesencephalon/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Neuropeptides/genetics*
  • Neuropeptides/metabolism
  • Preoptic Area/metabolism
  • Neurons/metabolism*
  • Brain/metabolism*
  • In Situ Hybridization
  • In Situ Hybridization, Fluorescence
(all 26)
PubMed
30903017 Full text @ Sci. Rep.
Abstract
Spexin (SPX) is a highly conserved neuropeptide that is widely expressed in mammalian brain and peripheral tissue. In teleost, SPX1 is mainly expressed in the brain and ovary, and is involved in reproduction and food intake. A second form of SPX, SPX2, was recently identified in chick, Xenopus, and zebrafish. The expression pattern and roles of SPX2 are unknown. SPX (spx1) is highly expressed in the vertebrate brain, but its distribution, circuits, and interactions with its putative receptor are unknown. Here, we observed expression of spx1 in the midbrain and hindbrain, and spx2 in the hypothalamic preoptic area using in situ RNA hybridization in zebrafish. Analysis of transgenic reporter zebrafish revealed that hindbrain SPX1 neurons are PAX2+ inhibitory interneurons and project to the spinal cord, where they interact with galanin receptor 2b (GALR2b) neurons, suggesting that hindbrain SPX1 neurons are reticulospinal neurons. spx1 mRNA and SPX1 reporter expression were observed in dorsal habenula (dHb). SPX1 neurons in the dHb project to the interpeduncular nucleus (IPN), where GALR2a and GALR2b expression was also observed, suggesting that habenula SPX1 neurons may interact with GALR2a/2b in the IPN.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cf3TgTransgenic Insertion
    km7TgTransgenic Insertion
      km8TgTransgenic Insertion
        km9TgTransgenic Insertion
          nkuasgfp1aTgTransgenic Insertion
            nns1TgTransgenic Insertion
              nns25TgTransgenic Insertion
                rw0TgTransgenic Insertion
                  rw0110bTgTransgenic Insertion
                    rw0135TgTransgenic Insertion
                      1 - 10 of 12
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      1 - 6 of 6
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                      Mapping
                      No data available