PUBLICATION

Midbrain tectal stem cells display diverse regenerative capacities in zebrafish

Authors
Lindsey, B.W., Aitken, G.E., Tang, J.K., Khabooshan, M., Douek, A.M., Vandestadt, C., Kaslin, J.
ID
ZDB-PUB-190316-4
Date
2019
Source
Scientific Reports   9: 4420 (Journal)
Registered Authors
Douek, Alon M., Kaslin, Jan
Keywords
none
MeSH Terms
  • Zebrafish
  • Animals, Genetically Modified
  • Tectum Mesencephali/cytology*
  • Tectum Mesencephali/physiology
  • Zebrafish Proteins/metabolism
  • Superior Colliculi/cytology*
  • Superior Colliculi/physiology
  • Cell Proliferation
  • Neuroglia/cytology*
  • Neuroglia/physiology
  • Neurons/cytology*
  • Neurons/physiology
  • Neurogenesis*
  • Wnt Signaling Pathway
  • Animals
  • Neural Stem Cells/cytology*
  • Neural Stem Cells/physiology
(all 17)
PubMed
30872640 Full text @ Sci. Rep.
Abstract
How diverse adult stem and progenitor populations regenerate tissue following damage to the brain is poorly understood. In highly regenerative vertebrates, such as zebrafish, radial-glia (RG) and neuro-epithelial-like (NE) stem/progenitor cells contribute to neuronal repair after injury. However, not all RG act as neural stem/progenitor cells during homeostasis in the zebrafish brain, questioning the role of quiescent RG (qRG) post-injury. To understand the function of qRG during regeneration, we performed a stab lesion in the adult midbrain tectum to target a population of homeostatic qRG, and investigated their proliferative behaviour, differentiation potential, and Wnt/β-catenin signalling. EdU-labelling showed a small number of proliferating qRG after injury (pRG) but that progeny are restricted to RG. However, injury promoted proliferation of NE progenitors in the internal tectal marginal zone (TMZi) resulting in amplified regenerative neurogenesis. Increased Wnt/β-catenin signalling was detected in TMZi after injury whereas homeostatic levels of Wnt/β-catenin signalling persisted in qRG/pRG. Attenuation of Wnt signalling suggested that the proliferative response post-injury was Wnt/β-catenin-independent. Our results demonstrate that qRG in the tectum have restricted capability in neuronal repair, highlighting that RG have diverse functions in the zebrafish brain. Furthermore, these findings suggest that endogenous stem cell compartments compensate lost tissue by amplifying homeostatic growth.
Genes / Markers
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Expression
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gl23TgTransgenic Insertion
    mi2001TgTransgenic Insertion
      w25TgTransgenic Insertion
        w32TgTransgenic Insertion
          y83TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
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            Fish
            No data available
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
            1 - 3 of 3
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            Mapping
            No data available
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            Citation
            Lindsey, B.W., Aitken, G.E., Tang, J.K., Khabooshan, M., Douek, A.M., Vandestadt, C., Kaslin, J. (2019) Midbrain tectal stem cells display diverse regenerative capacities in zebrafish. Scientific Reports. 9:4420.