PUBLICATION

bif1, a new BMP signaling inhibitor, regulates embryonic hematopoiesis in the zebrafish

Authors
Ghersi, J.J., Mahony, C.B., Bertrand, J.Y.
ID
ZDB-PUB-190308-4
Date
2019
Source
Development (Cambridge, England)   146(6): (Journal)
Registered Authors
Bertrand, Julien, Ghersi, Joey, Mahony, Christopher
Keywords
BMP signaling, Definitive hematopoiesis, Erythropoiesis, Hematopoietic Stem Cells, Zebrafish, Zinc finger proteins
MeSH Terms
  • Animals, Genetically Modified
  • Cell Lineage
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Erythropoiesis/genetics
  • Bone Morphogenetic Proteins/antagonists & inhibitors*
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Nucleus/metabolism
  • Hematopoiesis*
  • Signal Transduction
  • Green Fluorescent Proteins/metabolism
  • Gene Expression Regulation, Developmental*
  • Zinc Fingers
  • Zebrafish
  • Animals
  • Mice
  • Transcription Factors/metabolism
(all 17)
PubMed
30837221 Full text @ Development
Abstract
Hematopoiesis maintains the entire blood system and dysregulation of this process can lead to malignancies (leukemia), immunodeficiencies or red blood cell diseases (anemia, polycythemia vera). We took advantage of the zebrafish model that shares most of the genetic program involved in hematopoiesis with mammals to characterize a new gene of unknown function, si:ch73-299h12.2, expressed in the erythroid lineage during primitive, definitive and adult hematopoiesis. This gene, required during primitive and definitive erythropoiesis, encodes a C2H2 zinc finger protein that inhibits BMP signaling. We therefore named this gene: blood-inducing factor 1 and BMP inhibitory factor 1 (bif1). We identified a bif1 orthologue in Sinocyclocheilus Rhinocerous, another fish, and in the mouse genome. Both genes also inhibit BMP signaling when overexpressed in zebrafish. In conclusion, we deorphanized a new zebrafish gene of unknown function: bif1 codes for a zinc-finger protein that inhibits BMP signaling and also regulates primitive erythropoiesis and definitive hematopoiesis.
Genes / Markers
Figures
Figure Gallery (17 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-bif1.1standard conditionsPrim-5
AB + MO1-bif1.1standard conditionsPrim-5
AB + MO1-bif1.1standard conditionsLong-pec
AB + MO1-bif1.1standard conditionsProtruding-mouth
AB + MO1-bif1.1standard conditionsProtruding-mouth
AB + MO1-bif1.1standard conditionsDay 4
AB + MO1-bif1.1standard conditionsDay 4
AB + MO1-bif1.1standard conditionsDay 5
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39standard conditions5-9 somites
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39standard conditions5-9 somites
1 - 10 of 15
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m39
    		Deficiency
    mw29TgTransgenic Insertion
      sd2TgTransgenic Insertion
        ug105TgTransgenic Insertion
          ug106TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            bif1.1MO1-bif1.1MRPHLNO
            1 - 1 of 1
            Show
            Fish
            Fish
            AB
            AB + MO1-bif1.1
            Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39
            sd2Tg
            sd2Tg + MO1-bif1.1
            sd2Tg; ug105Tg
            ug106Tg
            1 - 7 of 7
            Show
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GFPEFGGFP
            TFPEFGTFP
            1 - 4 of 4
            Show
            Mapping
            No data available
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            Citation
            Ghersi, J.J., Mahony, C.B., Bertrand, J.Y. (2019) bif1, a new BMP signaling inhibitor, regulates embryonic hematopoiesis in the zebrafish. Development (Cambridge, England). 146(6):.