PUBLICATION

Germline Stem Cells Drive Ovary Regeneration in Zebrafish

Authors
Cao, Z., Mao, X., Luo, L.
ID
ZDB-PUB-190214-9
Date
2019
Source
Cell Reports   26: 1709-1717.e3 (Journal)
Registered Authors
Luo, Lingfei
Keywords
GSCs, Wnt, nanos2, ovary regeneration, zebrafish
MeSH Terms
  • Zebrafish
  • Germ Cells/metabolism*
  • Female
  • Animals
  • Ovary/metabolism*
PubMed
30759383 Full text @ Cell Rep.
Abstract
Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq41TgTransgenic Insertion
    pd1TgTransgenic Insertion
      w30TgTransgenic Insertion
        w32TgTransgenic Insertion
          zf3086TgTransgenic Insertion
            zf3087
              Small Deletion
              zf3088
                Small Deletion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                nanos2CRISPR1-nanos2CRISPR
                1 - 1 of 1
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                Fish
                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                Dendra2EFGDendra2
                EGFPEFGEGFP
                GFPEFGGFP
                NTREFGNTR
                1 - 4 of 4
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                Mapping
                No data available