PUBLICATION

Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline

Authors
Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., Sun, Y.
ID
ZDB-PUB-190124-3
Date
2019
Source
Marine biotechnology (New York, N.Y.)   21(2): 217-228 (Journal)
Registered Authors
He, Mudan, Sun, Yonghua, Xiong, Feng, Ye, Ding, Zhu, Zuoyan
Keywords
Oogenesis, Oogonia, Primordial germ cell, Spermatogenesis, Spermatogonia, piwil1
MeSH Terms
  • Female
  • Testis/growth & development
  • Gene Expression
  • Germ Cells/cytology
  • Animals, Genetically Modified
  • Ovary/growth & development
  • Embryonic Development*
  • Animals
  • Embryo, Nonmammalian/embryology
  • Sex Differentiation*
  • Male
  • Zebrafish Proteins
  • Luminescent Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics
(all 15)
PubMed
30671659 Full text @ Mar. Biotechnol.
Abstract
Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe the generation and characterization of a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)ihb327Tg, which specifically labels the whole lifetime of germ cells, i.e., from primordial germ cells (PGCs) at shield stage to the oogonia and early stage of oocytes in the ovary and to the early stage of spermatogonia, spermatocyte, and spermatid in the testis. By using this transgenic line, we carefully observed the numbers of PGCs from early embryonic stage to juvenile stage and the differentiation process of ovary and testis. The numbers of PGCs became variable at as early as 1 day post-fertilization (dpf). Interestingly, the embryos with a high amount of PGCs mainly developed into females and the ones with a low amount of PGCs mainly developed into males. By using transient overexpression and transgenic induction of PGC-specific bucky ball (buc), we further proved that induction of abundant PGCs at embryonic stage promoted later ovary differentiation and female development. Taken together, we generate an ideal transgenic line Tg(piwil1:egfp-UTRnanos3)ihb327Tg which can visualize zebrafish germline for a lifetime, and we have utilized this line to study germ cell development and gonad differentiation of teleost and to demonstrate that the increase of PGC number at embryonic stage promotes female differentiation.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ihb8TgTransgenic Insertion
    ihb120TgTransgenic Insertion
      ihb327TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        No data available
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab4-ddx4polyclonalIgGRabbit
        1 - 1 of 1
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        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        KALTA4EFGKALTA4
        1 - 2 of 2
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        Mapping
        No data available
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        Citation
        Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., Sun, Y. (2019) Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Marine biotechnology (New York, N.Y.). 21(2):217-228.