PUBLICATION

Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish

Authors
Hong, Q., Li, C., Ying, R., Lin, H., Li, J., Zhao, Y., Cheng, H., Zhou, R.
ID
ZDB-PUB-181228-6
Date
2018
Source
Protein & cell   10(5): 347-364 (Journal)
Registered Authors
Zhou, Rongjia
Keywords
Cyp19a1a, Sox3, apoptosis, follicle development, zebrafish
Datasets
GEO:GSE115806
MeSH Terms
  • Apoptosis
  • Aromatase/metabolism
  • Zebrafish Proteins/metabolism
  • Ovarian Follicle/growth & development*
  • Estradiol/metabolism*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Fertility/genetics
  • Fertility/physiology*
  • Animals
  • Female
  • Loss of Function Mutation
  • Ovary/physiology*
  • SOXB1 Transcription Factors/genetics
  • SOXB1 Transcription Factors/physiology*
(all 15)
PubMed
30588557 Full text @ Protein Cell
Abstract
Folliculogenesis is essential for production of female gametes in vertebrates. However, the molecular mechanisms underlying follicle development, particularly apoptosis regulation in ovary, remain elusive. Here, we generated sox3 knockout zebrafish lines using CRISPR/Cas9. sox3 knockout led to follicle development retardation and a reduced fecundity in females. Comparative analysis of transcriptome between sox3-/- and wild-type ovaries revealed that Sox3 was involved in pathways of ovarian steroidogenesis and apoptosis. Knockout of sox3 promoted follicle apoptosis and obvious apoptosis signals were detected in somatic cells of stages III and IV follicles of sox3-/- ovaries. Moreover, Sox3 can bind to and activate the promoter of cyp19a1a. Up-regulation of Cyp19a1a expression promoted 17β-estradiol synthesis, which inhibited apoptosis in follicle development. Thus, Sox3 functions as a regulator of Cyp19a1a expression, via 17β-E2 linking apoptosis suppression, which is implicated in improving female fecundity.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
whu101
    Small Deletion
    whu102
      Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      sox3CRISPR2-sox3CRISPR
      1 - 1 of 1
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      Fish
      1 - 3 of 3
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      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab3-cyp19a1polyclonal
        IgGRabbit
        Ab3-sox3polyclonalIgGRabbit
        Ab19-casp3polyclonal
          IgGRabbit
          1 - 3 of 3
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          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          No data available