PUBLICATION

Stereotypic generation of axial tenocytes from bipartite sclerotome domains in zebrafish

Authors
Ma, R.C., Jacobs, C.T., Sharma, P., Kocha, K.M., Huang, P.
ID
ZDB-PUB-181103-9
Date
2018
Source
PLoS Genetics   14: e1007775 (Journal)
Registered Authors
Huang, Peng, Jacobs, Craig, Kocha, Katrinka, Ma, Roger
Keywords
none
MeSH Terms
  • Body Patterning
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism
  • Animals
  • Signal Transduction
  • Tenocytes/cytology*
  • Tenocytes/metabolism
  • Tendons/embryology
  • Tendons/metabolism
  • Organogenesis
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Animals, Genetically Modified
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Muscles/embryology
  • Muscles/metabolism
  • Somites/embryology
(all 19)
PubMed
30388110 Full text @ PLoS Genet.
Abstract
Development of a functional musculoskeletal system requires coordinated generation of muscles, bones, and tendons. However, how axial tendon cells (tenocytes) are generated during embryo development is still poorly understood. Here, we show that axial tenocytes arise from the sclerotome in zebrafish. In contrast to mouse and chick, the zebrafish sclerotome consists of two separate domains: a ventral domain and a previously undescribed dorsal domain. While dispensable for sclerotome induction, Hedgehog (Hh) signaling is required for the migration and maintenance of sclerotome derived cells. Axial tenocytes are located along the myotendinous junction (MTJ), extending long cellular processes into the intersomitic space. Using time-lapse imaging, we show that both sclerotome domains contribute to tenocytes in a dynamic and stereotypic manner. Tenocytes along a given MTJ always arise from the sclerotome of the adjacent anterior somite. Inhibition of Hh signaling results in loss of tenocytes and enhanced sensitivity to muscle detachment. Together, our work shows that axial tenocytes in zebrafish originate from the sclerotome and are essential for maintaining muscle integrity.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a4598TgTransgenic Insertion
    c264TgTransgenic Insertion
      ca101TgTransgenic Insertion
        ca102TgTransgenic Insertion
          ca103TgTransgenic Insertion
            ca104TgTransgenic Insertion
              hu3072
                		Point Mutation
                s1999tTgTransgenic Insertion
                  ts294e
                    		Point Mutation
                    zf13TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab-A4.1025monoclonal
                        		IgG2aMouse
                        1 - 1 of 1
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        EGFPEFGEGFP
                        GAL4EFGGAL4
                        GCaMPEFGGCaMP
                        GFPEFGGFP
                        KaedeEFGKaede
                        mCherryEFGmCherry
                        NTREFGNTR
                        1 - 7 of 7
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                        Mapping
                        No data available
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                        Citation
                        Ma, R.C., Jacobs, C.T., Sharma, P., Kocha, K.M., Huang, P. (2018) Stereotypic generation of axial tenocytes from bipartite sclerotome domains in zebrafish. PLoS Genetics. 14:e1007775.