PUBLICATION

Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish

Authors
Mochizuki, T., Kojima, Y., Nishiwaki, Y., Harakuni, T., Masai, I.
ID
ZDB-PUB-181017-4
Date
2018
Source
Development (Cambridge, England)   145(20): (Journal)
Registered Authors
Harakuni, Tetsuya, Kojima, Yutaka, Masai, Ichiro, Mochizuki, Toshiaki, Nishiwaki, Yuko
Keywords
Endocytic trafficking, Lens fiber differentiation, VPS45, Zebrafish
MeSH Terms
  • Lens, Crystalline/cytology*
  • Lens, Crystalline/metabolism*
  • Integrin beta1/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
  • Epithelium/metabolism
  • Cell Differentiation*
  • Endocytosis*
  • Phenotype
  • Wnt Signaling Pathway
  • Zebrafish/metabolism*
  • Protein Transport
  • Mutation/genetics
  • Vesicular Transport Proteins/genetics
  • Vesicular Transport Proteins/metabolism*
  • Fibroblast Growth Factors/metabolism
  • Transforming Growth Factor beta/metabolism
(all 18)
PubMed
30322969 Full text @ Development
Abstract
In vertebrate lens, lens epithelial cells cover the anterior half of the lens fiber core. Lens epithelial cells proliferate, move posteriorly and start to differentiate into lens fiber cells at the lens equator. Although FGF signaling promotes this equatorial commencement of lens fiber differentiation, the underlying mechanism is not fully understood. Here, we show that lens epithelial cells abnormally enter lens fiber differentiation without passing through the equator in zebrafish vps45 mutants. VPS45 belongs to the Sec1/Munc18-like protein family and promotes endosome trafficking, which differentially modulates signal transduction. Ectopic lens fiber differentiation in vps45 mutants does not depend on FGF, but is mediated through activation of TGFβ signaling and inhibition of canonical Wnt signaling. Thus, VPS45 normally suppresses lens fiber differentiation in the anterior region of lens epithelium by modulating TGFβ and canonical Wnt signaling pathways. These data indicate a novel role of endosome trafficking to ensure equator-dependent commencement of lens fiber differentiation.
Genes / Markers
Figures
Figure Gallery (17 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment by environment: SB 505124Day 5
WTchemical treatment by environment: SB 505124Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
WTchemical treatment by environment: SU5402Day 5
vps45rw341/rw341standard conditionsLong-pec
1 - 10 of 125
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ca7TgTransgenic Insertion
    oki039TgTransgenic Insertion
      oki041TgTransgenic Insertion
        rw341
          		Insertion
          sa14216
            		Point Mutation
            w25TgTransgenic Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              rbsnMO1-rbsnMRPHLNO
              1 - 1 of 1
              Show
              Fish
              Fish
              oki039Tg
              oki041Tg
              vps45rw341/rw341
              vps45rw341/rw341; oki039Tg
              vps45rw341/rw341; oki041Tg
              WT
              1 - 6 of 6
              Show
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GFPEFGGFP
              1 - 2 of 2
              Show
              Mapping
              No data available
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              Citation
              Mochizuki, T., Kojima, Y., Nishiwaki, Y., Harakuni, T., Masai, I. (2018) Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish. Development (Cambridge, England). 145(20):.