PUBLICATION

Basal constriction during midbrain-hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase

Authors: Gutzman, J.H., Graeden, E., Brachmann, I., Yamazoe, S., Chen, J.K., Sive, H.
ID: ZDB-PUB-181012-11
Date: 2018
Source: Biology Open 7(11): (Journal)
Registered Authors: Brachmann, Isabel, Chen, James K., Gutzman, Jennifer, Sive, Hazel
Keywords: Basal constriction, Cell shape, Focal Adhesion Kinase, Midbrain-hindbrain boundary, Morphogenesis, Wnt5b, Zebrafish
MeSH Terms: none
PubMed: 30305282 Full text @ Biol. Open

Abstract

Basal constriction occurs at the zebrafish midbrain-hindbrain boundary constriction (MHBC) and is likely a widespread morphogenetic mechanism. 3D reconstruction demonstrates that MHBC cells are wedge-shaped, and initially constrict basally, with subsequent apical expansion. wnt5b is expressed in the MHB and is required for basal constriction. Consistent with a requirement for this pathway, expression of dominant negative Gsk3β overcomes wnt5b knockdown. Immunostaining identifies focal adhesion kinase (Fak) as active in the MHB region, and knockdown demonstrates Fak is a regulator of basal constriction. Tissue specific knockdown further indicates that Fak functions cell autonomously within the MHBC. Fak acts downstream of wnt5b, suggesting that Wnt5b signals locally as an early step in basal constriction and acts together with more widespread Fak activation. This study delineates signaling pathways that regulate basal constriction during brain morphogenesis.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
wnt5b	AB	control	14-19 somites	midbrain hindbrain boundary constriction	ISH	Fig. 2
wnt5b	AB	control	20-25 somites	midbrain hindbrain boundary constriction	ISH	Fig. 2
wnt5b	AB	control	Prim-5	midbrain hindbrain boundary constriction	ISH	Fig. 2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO2-wnt5b + MO4-tp53	control	Prim-5	midbrain hindbrain boundary constriction basal region increased width, abnormal	Fig. 2
AB + MO2-wnt5b + MO4-tp53	control	Prim-5	midbrain hindbrain boundary constriction basal region structure, abnormal	Fig. 2
AB + MO2-wnt5b + MO4-tp53	control	Prim-5	midbrain hindbrain boundary constriction epithelial cell morphogenesis process quality, abnormal	Fig. 2
AB + MO2-wnt5b + MO4-tp53	control	Prim-5	midbrain hindbrain boundary constriction midbrain-hindbrain boundary morphogenesis process quality, abnormal	Fig. 2
AB + MO4-tp53 + MO5-ptk2ab	control	Prim-5	midbrain hindbrain boundary constriction basal region increased width, abnormal	Fig. 3
AB + MO4-tp53 + MO5-ptk2ab	control	Prim-5	midbrain hindbrain boundary constriction basal region structure, abnormal	Fig. 3
AB + MO4-tp53 + MO5-ptk2ab	control	Prim-5	midbrain hindbrain boundary constriction epithelial cell morphogenesis process quality, abnormal	Fig. 3
AB + MO4-tp53 + MO5-ptk2ab	control	Prim-5	midbrain hindbrain boundary constriction midbrain-hindbrain boundary morphogenesis process quality, abnormal	Fig. 3
lamc1^m86/m86	control	Prim-5	midbrain hindbrain boundary constriction epithelial cell morphogenesis process quality, abnormal	Fig. S5
lamc1^m86/m86	control	Prim-5	midbrain hindbrain boundary constriction midbrain-hindbrain boundary morphogenesis process quality, abnormal	Fig. S5

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
m86		Point Mutation	lamc1

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ptk2ab	MO5-ptk2ab	MRPHLNO
ptk2ab	MO6-ptk2ab	MRPHLNO
tp53	MO4-tp53	MRPHLNO
wnt5b	MO2-wnt5b	MRPHLNO

Fish

Fish
AB
AB + MO2-wnt5b + MO4-tp53
AB + MO4-tp53 + MO5-ptk2ab
lamc1^m86/m86

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Gutzman et al., 2018 ZDB-PUB-181012-11 PMID:30305282

Basal constriction during midbrain-hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase

Gutzman et al., 2018

ZDB-PUB-181012-11
PMID:30305282