PUBLICATION

Ensheathing cells utilize dynamic tiling of neuronal somas in development and injury as early as neuronal differentiation

Authors
Nichols, E.L., Green, L.A., Smith, C.J.
ID
ZDB-PUB-180820-18
Date
2018
Source
Neural Development   13: 19 (Journal)
Registered Authors
Smith, Cody
Keywords
Development, Ensheathment, Neural niche, Neuronal soma, Tiling
MeSH Terms
  • Ganglia, Spinal/cytology
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish
  • Microscopy, Confocal
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Indoles/pharmacology
  • Animals, Genetically Modified
  • Peripheral Nerve Injuries/pathology
  • Peripheral Nerve Injuries/physiopathology*
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Neuroglia/cytology*
  • Neuroglia/physiology*
  • SOXE Transcription Factors/genetics
  • SOXE Transcription Factors/metabolism
  • Embryo, Nonmammalian
  • Sulfonamides/pharmacology
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Nerve Regeneration/genetics
  • Neurons/cytology*
  • Neurons/physiology*
(all 29)
PubMed
30121077 Full text @ Neural Dev.
Abstract
Background Glial cell ensheathment of specific components of neuronal circuits is essential for nervous system function. Although ensheathment of axonal segments of differentiated neurons has been investigated, ensheathment of neuronal cell somas, especially during early development when neurons are extending processes and progenitor populations are expanding, is still largely unknown.
Methods To address this, we used time-lapse imaging in zebrafish during the initial formation of the dorsal root ganglia (DRG).
Results Our results show that DRG neurons are ensheathed throughout their entire lifespan by a progenitor population. These ensheathing cells dynamically remodel during development to ensure axons can extend away from the neuronal cell soma into the CNS and out to the skin. As a population, ensheathing cells tile each DRG neuron to ensure neurons are tightly encased. In development and in experimental cell ablation paradigms, the oval shape of DRG neurons dynamically changes during partial unensheathment. During longer extended unensheathment neuronal soma shifting is observed. We further show the intimate relationship of these ensheathing cells with the neurons leads to immediate and choreographed responses to distal axonal damage to the neuron.
Conclusion We propose that the ensheathing cells dynamically contribute to the shape and position of neurons in the DRG by their remodeling activity during development and are primed to dynamically respond to injury of the neuron.
Genes / Markers
Figures
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
w18TgTransgenic Insertion
    w61TgTransgenic Insertion
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      Human Disease / Model
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      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab2-sox2polyclonalIgGRabbit
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      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      EosEFGEos
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      Citation
      Nichols, E.L., Green, L.A., Smith, C.J. (2018) Ensheathing cells utilize dynamic tiling of neuronal somas in development and injury as early as neuronal differentiation. Neural Development. 13:19.