PUBLICATION

Pitx2c orchestrates embryonic axis extension via mesendodermal cell migration

Authors
Collins, M.M., Maischein, H.M., Dufourcq, P., Charpentier, M., Blader, P., Stainier, D.Y.
ID
ZDB-PUB-180629-6
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Blader, Patrick, Dufourcq, Pascale, Maischein, Hans-Martin, Stainier, Didier
Keywords
Pitx2c, cell migration, chemokine signaling, developmental biology, gastrulation, integrin-fibronectin interaction, zebrafish
Datasets
GEO:GSE114671
MeSH Terms
  • Chemokines/genetics
  • Chemokines/metabolism
  • Cell Shape
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Embryonic Development/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Embryo, Nonmammalian
  • Zygote/cytology
  • Zygote/growth & development
  • Zygote/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Integrins/genetics
  • Integrins/metabolism
  • Body Patterning/genetics
  • Animals, Genetically Modified
  • Animals
  • Time-Lapse Imaging
  • Cell Movement/genetics*
  • Epithelial Cells/cytology
  • Epithelial Cells/metabolism*
  • Gene Expression Regulation, Developmental*
  • Transcriptome
  • Mutation
  • Notochord/cytology
  • Notochord/metabolism
  • Gastrulation/genetics
  • Endoderm/cytology
  • Endoderm/metabolism
(all 34)
PubMed
29952749 Full text @ Elife
Abstract
Pitx2c, a homeodomain transcription factor, is classically known for its left-right patterning role. However, an early wave of pitx2 expression occurs at the onset of gastrulation in several species, indicating a possible earlier role that remains relatively unexplored. Here we show that in zebrafish, maternal-zygotic (MZ) pitx2c mutants exhibit a shortened body axis indicative of convergence and extension (CE) defects. Live imaging reveals that MZpitx2c mutants display less persistent mesendodermal migration during late stages of gastrulation. Transplant data indicate that Pitx2c functions cell non-autonomously to regulate this cell behavior by modulating cell shape and protrusive activity. Using transcriptomic analyses and candidate gene approaches, we identify transcriptional changes in components of the chemokine-ECM-integrin dependent mesendodermal migration network. Together, our results define pathways downstream of Pitx2c that are required during early embryogenesis and reveal novel functions for Pitx2c as a regulator of morphogenesis.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
pitx2ups6/ups6standard conditionsShield
pitx2ups6/ups6standard conditionsShield
pitx2ups6/ups6standard conditionsShield
pitx2ups6/ups6standard conditionsShield to 90%-epiboly
pitx2ups6/ups6standard conditionsShield to 90%-epiboly
pitx2ups6/ups6standard conditions75%-epiboly
pitx2ups6/ups6standard conditions75%-epiboly
pitx2ups6/ups6standard conditions75%-epiboly
pitx2ups6/ups6standard conditions75%-epiboly
pitx2ups6/ups6standard conditions75%-epiboly
1 - 10 of 35
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s870TgTransgenic Insertion
    ups6
      		Indel
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      pitx2TALEN4-pitx2TALEN
      sox32MO1-sox32MRPHLNO
      1 - 2 of 2
      Show
      Fish
      Fish
      pitx2ups6/ups6
      pitx2ups6/ups6
      pitx2ups6/ups6; s870Tg
      s870Tg
      WT
      WT + MO1-sox32
      1 - 6 of 6
      Show
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      No data available
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      Citation
      Collins, M.M., Maischein, H.M., Dufourcq, P., Charpentier, M., Blader, P., Stainier, D.Y. (2018) Pitx2c orchestrates embryonic axis extension via mesendodermal cell migration. eLIFE. 7:.