PUBLICATION
Bsx controls pineal complex development
- Authors
- Schredelseker, T., Driever, W.
- ID
- ZDB-PUB-180628-2
- Date
- 2018
- Source
- Development (Cambridge, England) 145(13): (Journal)
- Registered Authors
- Driever, Wolfgang, Schredelseker, Theresa
- Keywords
- Brain asymmetry, Brain-specific homeobox transcription factor, Bsx, Epithalamus, Habenula, Melatonin, Parapineal organ, Photoreceptors, Pineal gland
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/genetics
- Homeodomain Proteins/biosynthesis*
- Homeodomain Proteins/genetics
- Gene Expression Regulation, Developmental/physiology*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- Melatonin/biosynthesis
- Melatonin/genetics
- Cell Differentiation/physiology*
- Pineal Gland/cytology
- Pineal Gland/embryology*
- Animals
- PubMed
- 29945867 Full text @ Development
Citation
Schredelseker, T., Driever, W. (2018) Bsx controls pineal complex development. Development (Cambridge, England). 145(13):.
Abstract
Neuroendocrine cells in the pineal gland release melatonin during the night and, in teleosts, are directly photoreceptive. During development of the pineal complex, a small number of cells migrate leftward away from the pineal anlage to form the parapineal cell cluster, a process that is crucial for asymmetrical development of the bilateral habenular nuclei. Here, we show that, throughout zebrafish embryonic development, the brain-specific homeobox (bsx) gene is expressed in all cell types of the pineal complex. We identified Bmp and Noto/Flh as major regulators of bsx expression in the pineal complex. Upon loss of Bsx through the generation of a targeted mutation, embryos fail to form a parapineal organ and develop right-isomerized habenulae. Crucial enzymes in the melatonin biosynthesis pathway are not expressed, suggesting the absence of melatonin from the pineal gland in bsx mutants. Several genes involved in rod-like or cone-like phototransduction are also abnormally expressed, indicating that Bsx has a pivotal role in the differentiation of multiple cell types in the zebrafish pineal complex.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping