PUBLICATION

Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry

Authors
Juan, T., Géminard, C., Coutelis, J.B., Cerezo, D., Polès, S., Noselli, S., Fürthauer, M.
ID
ZDB-PUB-180518-3
Date
2018
Source
Nature communications   9: 1942 (Journal)
Registered Authors
Fürthauer, Maximilian, Juan, Thomas, Polès, Sophie
Keywords
none
MeSH Terms
  • Animals
  • Myosins/genetics*
  • Myosins/metabolism
  • Left-Right Determination Factors/genetics
  • Left-Right Determination Factors/metabolism
  • Cilia/genetics
  • Cilia/metabolism
  • Gene Expression Regulation, Developmental
  • Body Patterning/genetics*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Animals, Genetically Modified
  • Mutation
  • Cell Polarity/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
(all 19)
PubMed
29769531 Full text @ Nat. Commun.
Abstract
The establishment of left-right (LR) asymmetry is fundamental to animal development, but the identification of a unifying mechanism establishing laterality across different phyla has remained elusive. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, LR asymmetry can be established independently of cilia, notably through the intrinsic chirality of the acto-myosin cytoskeleton. Here, we show that Myosin1D (Myo1D), a previously identified regulator of Drosophila LR asymmetry, is essential for the formation and function of the zebrafish LR organizer (LRO), Kupffer's vesicle (KV). Myo1D controls the orientation of LRO cilia and interacts functionally with the planar cell polarity (PCP) pathway component VanGogh-like2 (Vangl2), to shape a productive LRO flow. Our findings identify Myo1D as an evolutionarily conserved regulator of animal LR asymmetry, and show that functional interactions between Myo1D and PCP are central to the establishment of animal LR asymmetry.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment by environment: forskolin, chemical treatment by environment: 3-isobutyl-1-methylxanthine5-9 somites
dnaaf1tm317b/tm317bstandard conditionsLong-pec
myo1dnce16b/+standard conditionsAdult
myo1dnce16b/nce16bstandard conditions5-9 somites
myo1dnce16b/nce16bstandard conditions5-9 somites
myo1dnce16b/nce16bstandard conditions5-9 somites
myo1dnce16b/nce16bstandard conditions5-9 somites
myo1dnce16b/nce16bstandard conditions20-25 somites
myo1dnce16b/nce16bstandard conditionsPrim-5
myo1dnce16b/nce16bstandard conditionsPrim-5
1 - 10 of 50
Show
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
myo1dCRISPR1-myo1dCRISPR
myo1dMO2-myo1dMRPHLNO
myo1gCRISPR1-myo1gCRISPR
1 - 3 of 3
Show
Fish
Fish
AB/TU
dnaaf1tm317b/tm317b
ha01Tg + MO2-myo1d
myo1dnce16b/nce16b
myo1dnce16b/nce16b
myo1dnce16b/nce16b; myo1gnce18b/nce18b
myo1dnce16b/nce16b; vangl2m209/+
myo1dnce16b/+
myo1dnce16c/nce16c
myo1gsa17276/sa17276
1 - 10 of 13
Show
Antibodies
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
mRFPEFGmRFP
1 - 2 of 2
Show
Mapping
No data available
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Citation
Juan, T., Géminard, C., Coutelis, J.B., Cerezo, D., Polès, S., Noselli, S., Fürthauer, M. (2018) Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry. Nature communications. 9:1942.