PUBLICATION

Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene

Authors
Nikaido, M., Izumi, S., Ohnuki, H., Takigawa, Y., Yamasu, K., Hatta, K.
ID
ZDB-PUB-180208-11
Date
2018
Source
Gene expression patterns : GEP   28: 12-21 (Journal)
Registered Authors
Hatta, Kohei, Nikaido, Masataka, Yamasu, Kyo
Keywords
none
MeSH Terms
  • Choline O-Acetyltransferase/genetics*
  • Organogenesis
  • Animals
  • Regulatory Sequences, Nucleic Acid*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/physiology
  • Cell Differentiation
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development*
  • Animals, Genetically Modified/physiology
  • Cell Movement
  • Enteric Nervous System/growth & development*
  • Enteric Nervous System/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism*
  • Gene Expression Regulation, Developmental*
(all 19)
PubMed
29413438 Full text @ Gene Expr. Patterns
Abstract
The enteric nervous system (ENS) is the largest part of the peripheral nervous system in vertebrates. Toward the visualization of the development of the vertebrate ENS, we report our creation of a new transgenic line, Tg(chata:GGFF2) which has a 1.5-kb upstream region of the zebrafish choline acetyltransferase a (chata) gene followed by modified green fluorescent protein (gfp). During development, GFP + cells were detected in the gut by 60 h post-fertilization (hpf). In the gut of 6- and 12-days post-fertilization (dpf) larvae, an average of 92% of the GFP + cells were positive for the neuronal marker HuC/D, suggesting that GFP marks enteric neurons in this transgenic line. We also observed that 66% of the GFP + cells were choline acetyltransferase (ChAT)-immunopositive at 1.5 months. Thus, GFP is expressed at the larval stages at which ChAT protein expression is not yet detected by immunostaining. We studied the spatiotemporal pattern of neural differentiation in the ENS by live-imaging of this transgenic line. We observed that GFP + or gfp + cells initially formed a pair of bilateral rows at 60 hpf or 53 hpf, respectively, in the migrating enteric neural crest cells. Most of the GFP + cells did not migrate, and most of the new GFP + cells were added to fill the space among the previously formed GFP + cells. GFP expression reached the anus by 72 hpf. New GFP + cells then also appeared in the dorsal and ventral sides of the initial GFP + rows, resulting in their distribution on the entire gut by 4 dpf. A small number of new GFP + cells were found to move among older GFP + cells just before the cells stopped migration, suggesting that the moving GFP + cells may represent neural precursor cells searching for a place for the final differentiation. Our data suggest that the Tg(chata:GGFF2) line could serve as a useful tool for studies of enteric neural differentiation and cell behavior.
Genes / Markers
Figures
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Expression
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf2030TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
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    Fish
    No data available
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GAL4FFEFGGAL4FF
    GFPEFGGFP
    1 - 2 of 2
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    Mapping
    No data available
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    Citation
    Nikaido, M., Izumi, S., Ohnuki, H., Takigawa, Y., Yamasu, K., Hatta, K. (2018) Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene. Gene expression patterns : GEP. 28:12-21.