PUBLICATION

sox2 and sox3 play unique roles in development of hair cells and neurons in the zebrafish inner ear

Authors
Gou, Y., Vemaraju, S., Sweet, E.M., Kwon, H.J., Riley, B.B.
ID
ZDB-PUB-180123-5
Date
2018
Source
Developmental Biology   435(1): 73-83 (Journal)
Registered Authors
Kwon, Hye-Joo, Riley, Bruce, Sweet, Elly, Vemaraju, Shruti
Keywords
SoxB1, otic placode, sensory epithelia, statoacoustic ganglion
MeSH Terms
  • PAX2 Transcription Factor/genetics
  • PAX2 Transcription Factor/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Neurogenesis/physiology*
  • Zebrafish/embryology*
  • Gene Expression Regulation, Developmental/physiology*
  • SOX Transcription Factors/biosynthesis*
  • SOX Transcription Factors/genetics
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Animals
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Hair Cells, Auditory, Inner/cytology
  • Hair Cells, Auditory, Inner/metabolism*
(all 17)
PubMed
29355523 Full text @ Dev. Biol.
Abstract
Formation of neural and sensory progenitors in the inner ear requires Sox2 in mammals, and in other species is thought to rely on both Sox2 and Sox3. How Sox2 and/or Sox3 promote different fates is poorly understood. Our mutant analysis in zebrafish showed that sox2 is uniquely required for sensory development while sox3 is uniquely required for neurogenesis. Moderate misexpression of sox2 during placodal stages led to development of otic vesicles with expanded sensory and reduced neurogenic domains. However, high-level misexpression of sox2 or sox3 expanded both sensory and neurogenic domains to fill the medial and lateral halves of the otic vesicle, respectively. Disruption of medial factor pax2a eliminated the ability of sox2/3 misexpression to expand sensory but not neurogenic domains. Additionally, mild misexpression of fgf8 during placodal development was sufficient to specifically expand the zone of prosensory competence. Later, cross-repression between atoh1a and neurog1 helps maintain the sensory-neural boundary, but unlike mouse this does not require Notch activity. Together, these data show that sox2 and sox3 exhibit intrinsic differences in promoting sensory vs. neural competence, but at high levels these factors can mimic each other to enhance both states. Regional cofactors like pax2a and fgf8 also modify sox2/3 functions.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s356tTgTransgenic Insertion
    ta52b
      Point Mutation
      x17TgTransgenic Insertion
        x20TgTransgenic Insertion
          x21TgTransgenic Insertion
            x28TgTransgenic Insertion
              x32TgTransgenic Insertion
                x50
                  Small Deletion
                  x52
                    Small Deletion
                    1 - 9 of 9
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    pax2aMO1-pax2aMRPHLNO
                    tp53MO4-tp53MRPHLNO
                    1 - 2 of 2
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                    Fish
                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    GFPEFGGFP
                    1 - 1 of 1
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                    Mapping
                    No data available