PUBLICATION

Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1

Authors

Kimelman, D., Smith, N.L., Lai, J.K.H., Stainier, D.Y.

ID

ZDB-PUB-171229-4

Date

2017

Source

eLIFE 6: (Journal)

Registered Authors

Kimelman, David, Stainier, Didier

Keywords

developmental biology, stem cells, zebrafish

Datasets

GEO:GSE102606

MeSH Terms

Morphogenesis*
Zebrafish/embryology*
Zebrafish Proteins/metabolism*
Animals
Gene Expression Regulation, Developmental*
Trans-Activators/metabolism*
Intracellular Signaling Peptides and Proteins/metabolism*
Notochord/embryology*
Epidermis/embryology*
Fibronectins/metabolism

(all 10)

PubMed

29283341 Full text @ Elife

Abstract

The vertebrate embryo undergoes a series of dramatic morphological changes as the body extends to form the complete anterior-posterior axis during the somite-forming stages. The molecular mechanisms regulating these complex processes are still largely unknown. We show that the Hippo pathway transcriptional coactivators Yap1 and Wwtr1 are specifically localized to the presumptive epidermis and notochord, and play a critical and unexpected role in posterior body extension by regulating Fibronectin assembly underneath the presumptive epidermis and surrounding the notochord. We further find that Yap1 and Wwtr1, also via Fibronectin, have an essential role in the epidermal morphogenesis necessary to form the initial dorsal and ventral fins, a process previously thought to involve bending of an epithelial sheet, but which we now show involves concerted active cell movement. Our results reveal how the Hippo pathway transcriptional program, localized to two specific tissues, acts to control essential morphological events in the vertebrate embryo.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
wwtr1^bns35/bns35	control	14-19 somites	ectoderm wwtr1 expression absent, abnormal	Fig. 2-S2
wwtr1^bns35/bns35	control	14-19 somites	notochord wwtr1 expression absent, abnormal	Fig. 2-S2
yap1^bns19/bns19	control	14-19 somites	ectoderm yap1 expression absent, abnormal	Fig. 2
yap1^bns19/bns19	control	14-19 somites	notochord yap1 expression absent, abnormal	Fig. 2
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	ectoderm ecrg4b expression absent, abnormal	Fig. 3
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	ectoderm anatomical region ab2-fn labeling spatial pattern, abnormal	Fig. 9
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	ectoderm separated from somite, abnormal	Fig. 11
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	notochord posterior region wu:fc23c09 expression absent, abnormal	Fig. 3-S1
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	notochord posterior region ab2-fn labeling decreased amount, abnormal	Fig. 9
yap1^bns19/bns19; wwtr1^bns35/bns35	control	14-19 somites	notochord undulate, abnormal	Fig. 9

1 - 10 of 26

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
bns19		Small Deletion	yap1
bns35		Insertion	wwtr1
n1		Small Deletion	noto
zf621Tg	Tg(hsp70l:RFP-dnyap1)	Transgenic Insertion
zf622Tg	Tg(hsp70l:RFP-cayap1)	Transgenic Insertion

1 - 5 of 5

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Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
RFP	EFG	RFP

Mapping

Kimelman et al., 2017 ZDB-PUB-171229-4 PMID:29283341

Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1

Kimelman et al., 2017

ZDB-PUB-171229-4
PMID:29283341