PUBLICATION

Lineage tracing of dlx1a/2a and dlx5a/6a expressing cells in the developing zebrafish brain

Authors
Solek, C.M., Feng, S., Perin, S., Weinschutz Mendes, H.C., Ekker, M.
ID
ZDB-PUB-170510-12
Date
2017
Source
Developmental Biology   427(1): 131-147 (Journal)
Registered Authors
Ekker, Marc
Keywords
Dlx, GABA, lineage tracing, zebrafish
MeSH Terms
  • Animals
  • Microscopy, Fluorescence
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • GABAergic Neurons/cytology
  • GABAergic Neurons/metabolism*
  • Brain/cytology
  • Brain/embryology
  • Brain/metabolism*
  • Mice
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Microscopy, Confocal
  • In Situ Hybridization
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Mice, Transgenic
  • Immunohistochemistry
  • Cell Lineage/genetics
  • Mouse Embryonic Stem Cells/cytology
  • Mouse Embryonic Stem Cells/metabolism*
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/embryology
  • Embryo, Mammalian/metabolism
(all 29)
PubMed
28479339 Full text @ Dev. Biol.
Abstract
Lineage tracing of specific populations of progenitor cells provides crucial information about developmental programs. Four members of the Dlx homeobox gene family, Dlx1,2, 5 and 6, are involved in the specification of γ-aminobutyric acid (GABA)ergic neurons in the vertebrate forebrain. Orthologous genes in mammals and teleost show similarities in expression patterns and transcriptional regulation mechanisms. We have used lineage tracing to permanently label dlx-expressing cells in the zebrafish and have characterized the progeny of these cells in the larva and in the juvenile and adult brain. We have found that dlx1a/2a and dlx5a/6a expressing progenitors give rise, for the most part, to small populations of cells which constitute only a small proportion of GABAergic cells in the adult brain tissue. Moreover, some of the cells do not acquire a neuronal phenotype suggesting that, regardless of the time a cell expresses dlx genes in the brain, it can potentially give rise to cells other than neurons. In some instances, labeling larval dlx5a/6a-expressing cells, but not dlx1a/2a-expressing cells, results in massively expanding, widespread clonal expansion throughout the adult brain. Our data provide a detailed lineage analysis of the dlx1a/2a and dlx5a/6a expressing progenitors in the zebrafish brain and lays the foundation for further characterization of the role of these transcription factors beyond the specification of GABAergic neurons.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cz1701TgTransgenic Insertion
    ot20TgTransgenic Insertion
      ot60TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        No data available
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        CreEFGCre
        EGFPEFGEGFP
        mCherryEFGmCherry
        1 - 3 of 3
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        Mapping
        No data available