PUBLICATION

Dynamic regulation of Pin1 expression and function during zebrafish development

Authors

Ibarra, M.S., Borini Etichetti, C., Di Benedetto, C., Rosano, G.L., Margarit, E., Del Sal, G., Mione, M., Girardini, J.

ID

ZDB-PUB-170421-3

Date

2017

Source

PLoS One 12: e0175939 (Journal)

Registered Authors

Mione, Marina

Keywords

Embryos, Zebrafish, Immunofluorescence, Phosphorylation, Apoptosis, Messenger RNA, Subcellular localization, Mouse models

MeSH Terms

Animals
Gene Expression Regulation, Developmental*
Zebrafish/embryology*
Substrate Specificity
Gene Expression Regulation, Enzymologic*
NIMA-Interacting Peptidylprolyl Isomerase/genetics
NIMA-Interacting Peptidylprolyl Isomerase/metabolism*

(all 7)

PubMed

28426725 Full text @ PLoS One

Abstract

The prolyl isomerase Pin1 plays a key role in the modulation of proline-directed phosphorylation signaling by inducing local conformational changes in phosphorylated protein substrates. Extensive studies showed different roles for Pin1 in physiological processes and pathological conditions such as cancer and neurodegenerative diseases. However, there are still several unanswered questions regarding its biological role. Notably, despite evidences from cultured cells showing that Pin1 expression and activity may be regulated by different mechanisms, little is known on their relevance in vivo. Using Danio rerio (zebrafish) as a vertebrate model organism we showed that pin1 expression is regulated during embryogenesis to achieve specific mRNA and protein distribution patterns. Moreover, we found different subcellular distribution in particular stages and cell types and we extended the study of Pin1 expression to the adult zebrafish brain. The analysis of Pin1 overexpression showed alterations on zebrafish development and the presence of p53-dependent apoptosis. Collectively, our results suggest that specific mechanisms are operated in different cell types to regulate Pin1 function.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
pin1	AB	standard conditions	Adult	central caudal thalamic nucleus	IHC	Fig. 6
pin1	AB	standard conditions	Adult	cerebellum	IHC	Fig. 6
pin1	AB	standard conditions	Long-pec	cerebellum	IHC	Fig. 3
pin1	AB	standard conditions	Long-pec	cerebellum	ISH	Fig. 1
pin1	AB	standard conditions	Long-pec	cerebellum	IHC	Fig. S3
pin1	AB	standard conditions	Prim-5	cerebellum	IHC	Fig. 3
pin1	AB	standard conditions	Prim-5	cerebellum	ISH	Fig. 1
pin1	AB	standard conditions	Long-pec	diencephalon ventricular zone	ISH	Fig. 1
pin1	AB	standard conditions	Prim-5	diencephalon ventricular zone	ISH	Fig. 1
pin1	AB	standard conditions	Prim-5	diencephalon	IHC	Fig. 3

1 - 10 of 77

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-pin1	standard conditions	Prim-5	whole organism pin1 expression decreased amount, abnormal	Fig. S2

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zdf1		Point Mutation	tp53

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
pin1	MO1-pin1	MRPHLNO

Fish

Fish
AB
AB + MO1-pin1

Antibodies

Orthology

Gene	Orthology
pin1

Engineered Foreign Genes

Mapping

Ibarra et al., 2017 ZDB-PUB-170421-3 PMID:28426725

Dynamic regulation of Pin1 expression and function during zebrafish development

Ibarra et al., 2017

ZDB-PUB-170421-3
PMID:28426725