PUBLICATION

Dynamic regulation of Pin1 expression and function during zebrafish development

Authors
Ibarra, M.S., Borini Etichetti, C., Di Benedetto, C., Rosano, G.L., Margarit, E., Del Sal, G., Mione, M., Girardini, J.
ID
ZDB-PUB-170421-3
Date
2017
Source
PLoS One   12: e0175939 (Journal)
Registered Authors
Mione, Marina
Keywords
Embryos, Zebrafish, Immunofluorescence, Phosphorylation, Apoptosis, Messenger RNA, Subcellular localization, Mouse models
MeSH Terms
  • Animals
  • Substrate Specificity
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Developmental*
  • NIMA-Interacting Peptidylprolyl Isomerase/genetics
  • NIMA-Interacting Peptidylprolyl Isomerase/metabolism*
  • Zebrafish/embryology*
(all 7)
PubMed
28426725 Full text @ PLoS One
Abstract
The prolyl isomerase Pin1 plays a key role in the modulation of proline-directed phosphorylation signaling by inducing local conformational changes in phosphorylated protein substrates. Extensive studies showed different roles for Pin1 in physiological processes and pathological conditions such as cancer and neurodegenerative diseases. However, there are still several unanswered questions regarding its biological role. Notably, despite evidences from cultured cells showing that Pin1 expression and activity may be regulated by different mechanisms, little is known on their relevance in vivo. Using Danio rerio (zebrafish) as a vertebrate model organism we showed that pin1 expression is regulated during embryogenesis to achieve specific mRNA and protein distribution patterns. Moreover, we found different subcellular distribution in particular stages and cell types and we extended the study of Pin1 expression to the adult zebrafish brain. The analysis of Pin1 overexpression showed alterations on zebrafish development and the presence of p53-dependent apoptosis. Collectively, our results suggest that specific mechanisms are operated in different cell types to regulate Pin1 function.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zdf1
    Point Mutation
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    pin1MO1-pin1MRPHLNO
    1 - 1 of 1
    Show
    Fish
    1 - 2 of 2
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    Antibodies
    Orthology
    Gene Orthology
    pin1
    1 - 1 of 1
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    Engineered Foreign Genes
    No data available
    Mapping
    No data available