PUBLICATION

MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos

Authors
Kasper, D.M., Moro, A., Ristori, E., Narayanan, A., Hill-Teran, G., Fleming, E., Moreno-Mateos, M., Vejnar, C.E., Zhang, J., Lee, D., Gu, M., Gerstein, M., Giraldez, A., Nicoli, S.
ID
ZDB-PUB-170330-6
Date
2017
Source
Developmental Cell   40: 552-565.e5 (Journal)
Registered Authors
Giraldez, Antonio, Kasper, Dionna, Moro, Albertomaria, Nicoli, Stefania, Ristori, Emma, Vejnar, Charles
Keywords
cardiovascular development, endothelial cells, environmental stress, miR-139, miR-223, miR-24, phenotypic variability, robustness, target gene networks, zebrafish mutants
Datasets
GEO:GSE81335, GEO:GSE81341, GEO:GSE81340
MeSH Terms
  • Arteries/embryology
  • Arteries/metabolism
  • Gene Regulatory Networks
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Morphogenesis
  • Mutation/genetics
  • Homozygote
  • Vertebrates/embryology*
  • Vertebrates/genetics*
  • Genome
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Cell Count
  • Embryo, Nonmammalian/metabolism*
  • Endothelial Cells/metabolism
  • Animals
  • Pseudopodia/metabolism
  • Quantitative Trait, Heritable
  • Phenotype
  • Stress, Physiological
  • Mutagenesis/genetics
(all 24)
PubMed
28350988 Full text @ Dev. Cell
Abstract
Proper functioning of an organism requires cells and tissues to behave in uniform, well-organized ways. How this optimum of phenotypes is achieved during the development of vertebrates is unclear. Here, we carried out a multi-faceted and single-cell resolution screen of zebrafish embryonic blood vessels upon mutagenesis of single and multi-gene microRNA (miRNA) families. We found that embryos lacking particular miRNA-dependent signaling pathways develop a vascular trait similar to wild-type, but with a profound increase in phenotypic heterogeneity. Aberrant trait variance in miRNA mutant embryos uniquely sensitizes their vascular system to environmental perturbations. We discovered a previously unrecognized role for specific vertebrate miRNAs to protect tissue development against phenotypic variability. This discovery marks an important advance in our comprehension of how miRNAs function in the development of higher organisms.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la116TgTransgenic Insertion
    s896TgTransgenic Insertion
      ya302
        Small Deletion
        ya303
          Small Deletion
          ya304
            Small Deletion
            ya324
              Indel
              ya325
                Indel
                ya326
                  Small Deletion
                  ya327
                    Small Deletion
                    zf169TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GFPEFGGFP
                      mCherryEFGmCherry
                      1 - 3 of 3
                      Show
                      Mapping
                      No data available